Effect of fasting and thermal acclimation on metabolism of juvenile axolotls(Ambystoma mexicanum)
Effect of fasting and thermal acclimation on metabolism of juvenile axolotls(Ambystoma mexicanum)
复制标题
禁食和热驯化对墨西哥钝口螈幼鱼代谢的影响
DOI：
10.1007/978-3-642-60083-8_1
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
D. A. Caruso
中科院分区：
文献类型：
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作者：
L. Irwin;Karen A. Talentino;D. A. Caruso
让幼年蝾螈适应 15°C 或 25°C 的温度，并在这两种温度下进食或禁食，以研究热适应和营养状态对新陈代谢的相互作用。在 15℃ 时，禁食显着增加了耗氧量，但热适应没有显着增加。禁食还增加了两种氧化代谢酶（柠檬酸合酶和细胞色素氧化酶）的比活性，但不增加糖酵解酶乳酸脱氢酶的比活性。冷驯化进一步刺激了细胞色素氧化酶的比活性。禁食动物中甘油三酯和脂肪酸严重消耗，但热适应对脂质储存没有显着影响。这项研究说明：（1）禁食后各种代谢反应的不同性质； (2) 变温动物热驯化实验中营养状态的混杂相互作用。
Juvenile axolotls were acclimated to 15○C or 25○C and either fed or fasted at both temperatures, to study the interaction of thermal acclimation and nutritional state on metabolism. Fasting but not thermal acclimation significantly increased oxygen consumption at 15○C. Fasting also increased the specific activities of two oxidative metabolic enzymes – citrate synthase and cytochrome oxidase – but not that of the glycolytic enzyme lactic dehydrogenase. The specific activity of cytochrome oxidase was further stimulated by cold acclimation. Triglycerides and fatty acids were severely depleted in fasted animals, but thermal acclimation had no significant effect on lipid stores. This study illustrates: (1) the differential nature of various metabolic responses to fasting; and (2) the confounding interaction of the nutritional state on thermal acclimation experiments in an ectotherm.