3D Acoustic Wave Sparsely Activated Localization Microscopy With Phase Change Contrast Agents
3D Acoustic Wave Sparsely Activated Localization Microscopy With Phase Change Contrast Agents
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DOI：
10.1097/rli.0000000000001033
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发表时间：
2024-05-01
影响因子：
6.7
通讯作者：
Tang，Meng-Xing
中科院分区：
文献类型：
--
作者：
Riemer，Kai;Tan，Qingyuan;Tang，Meng-Xing
本研究的目的是证明三维（3D）声波稀疏激活定位显微镜（AWSALM）的微血管流动在体内使用相变造影剂（PCCAs.Materials and MethodsThree-dimensional AWSALM使用声激活PCCAs进行了评估交叉管微流体模，新西兰白色兔的肾脏，C57 BL/6 J小鼠的大脑通过完整的头骨。采用C3 F8和C4 F10低沸点氟碳化合物混合气体，在合适的活化压力下制备PCCA。连接到256通道超声研究平台的多路复用8 MHz矩阵阵列用于传输激活和成像超声脉冲并记录回波。随后，在体外和体内的回波数据进行波束形成和处理，使用一组定制的算法生成3D超分辨率超声图像，通过定位和跟踪激活contrast agents.ResultsWith 3D AWSALM，PCCA的声学激活可以控制空间和时间，使对比度的需求，并能够揭示3D微血管连通性。利用傅里叶壳层相关测量的三维AWSALM图像的空间分辨率为64 μm，与点扩散函数相比提高了9倍，与半波长相比提高了1.5倍。与基于微泡的方法相比，更多的信号以相似的浓度定位在微血管中，同时在较大的血管中保持稀疏和较长的轨迹。经颅成像被证明是一个证明的原则PCCA激活在小鼠大脑与3D AWSALM.ConclusionsThree-dimensional AWSALM生成体积超声超分辨率微血管图像在体内的时空选择性和增强微血管渗透。
ObjectiveThe aim of this study is to demonstrate 3-dimensional (3D) acoustic wave sparsely activated localization microscopy (AWSALM) of microvascular flow in vivo using phase change contrast agents (PCCAs).Materials and MethodsThree-dimensional AWSALM using acoustically activable PCCAs was evaluated on a crossed tube microflow phantom, the kidney of New Zealand White rabbits, and the brain of C57BL/6J mice through intact skull. A mixture of C 3 F 8 and C 4 F 10 low-boiling-point fluorocarbon gas was used to generate PCCAs with an appropriate activation pressure. A multiplexed 8-MHz matrix array connected to a 256-channel ultrasound research platform was used for transmitting activation and imaging ultrasound pulses and recording echoes. The in vitro and in vivo echo data were subsequently beamformed and processed using a set of customized algorithms for generating 3D super-resolution ultrasound images through localizing and tracking activated contrast agents.ResultsWith 3D AWSALM, the acoustic activation of PCCAs can be controlled both spatially and temporally, enabling contrast on demand and capable of revealing 3D microvascular connectivity. The spatial resolution of the 3D AWSALM images measured using Fourier shell correlation is 64 μm, presenting a 9-time improvement compared with the point spread function and 1.5 times compared with half the wavelength. Compared with the microbubble-based approach, more signals were localized in the microvasculature at similar concentrations while retaining sparsity and longer tracks in larger vessels. Transcranial imaging was demonstrated as a proof of principle of PCCA activation in the mouse brain with 3D AWSALM.ConclusionsThree-dimensional AWSALM generates volumetric ultrasound super-resolution microvascular images in vivo with spatiotemporal selectivity and enhanced microvascular penetration.