Periosteum-derived cells respond to mechanical stretch and activate Wnt and BMP signaling pathways
Periosteum-derived cells respond to mechanical stretch and activate Wnt and BMP signaling pathways
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DOI：
10.2220/biomedres.35.69
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发表时间：
2014-02-01
影响因子：
1.2
通讯作者：
Kimura, Hiroto
中科院分区：
文献类型：
--
作者：
Ito, Ryohei;Matsumiya, Tomoh;Kimura, Hiroto
骨膜为骨代谢提供成骨细胞和营养物质，并且对于成骨细胞分化和骨生成是重要的。近年来，骨膜来源的细胞已被用于口面部骨再生治疗。然而，骨膜在生理性骨重建中的作用却知之甚少。在我们的假设，骨膜感觉到机械应力，以诱导骨重建，我们进行人颌骨骨膜细胞（HJBPCs）单轴拉伸24小时，其特征在于其基因表达谱的微阵列分析。在检测到的62,976个基因中，提取了550个与骨代谢相关的基因，这些基因表达变化较大的基因中有76个入围。结果表明，在HJBPCs的机械拉伸调节基因的表达水平参与无翅型MMTV整合（Wnt）网站，骨形态发生蛋白（BMP）信号通路，和炎症细胞因子。我们认为骨膜来源的细胞感知机械应力，然后激活和调节成骨细胞分化和成骨的信号。
The periosteum supplies osteoblasts and nutrients for bone metabolism and is important for osteoblast differentiation and osteogenesis. Recently, periosteum-derived cells have been used for orofacial bone regeneration therapy. However, little is known about the function of the periosteum in physiological bone remodeling. On our hypothesis that the periosteum senses a mechanical stress to induce bone remodeling, we subjected human jaw bone periosteum cells (HJBPCs) to uniaxial stretching for 24 h and characterized their gene expression profiles by microarray analysis. Of 62,976 genes detected, 550 genes related to bone metabolism were extracted, and 76 of these genes with large changes in gene expression were short-listed. The results indicated that mechanical stretch in HJBPCs regulated the expression levels of genes involved in the Wingless-type MMTV integration (Wnt) site, bone morphogenetic protein (BMP) signaling pathways, and inflammatory cytokines. We propose that periosteum-derived cells sense mechanical stress and then activate and regulate signals for osteoblast differentiation and osteogenesis.