The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes
The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes
复制标题
微重力引起纤毛缩短和骨细胞顺行/逆行鞭毛内运输比例增加
DOI：
10.1016/j.bbrc.2020.06.119
复制
发表时间：
2020-09-10
影响因子：
3.1
通讯作者：
Fan, Yu-bo
中科院分区：
文献类型：
--
作者：
Ding, Dong;Yang, Xiao;Fan, Yu-bo
很难解释在微重力下骨细胞力学敏感性降低的原因。初级纤毛是骨细胞必不可少的机械传感器。在模拟微重力环境下，纤毛变短。纤毛的改变可能是SMG作用下骨细胞力学敏感性降低的原因。为了揭示初级纤毛在失重诱导的骨细胞功能障碍中的作用，我们研究了鞭毛内运输（IFT），以了解在SMG作用下骨细胞纤毛长度减少的机制。我们用GFP::IFT88测量顺行IFT粒子的数量，用OFP::IFT43测量逆行IFT粒子的数量，它们出现在纤毛的特定横切面上。我们还测量了SMG下IFT88和IFT43的表达以及IFT颗粒的大小。其中，SMG作用下，顺行/逆行颗粒数之比和IFT88/IFT43蛋白表达比增加。在SMG作用下，GFP::IFT88顺行IFT颗粒的大小显著减小。SMG从根本上打破了IFT的平衡运行状态，使IFT颗粒变小。SMG下的现象很有趣。(c) 2020 Elsevier Inc.版权所有。
It is hard to explain the decrease in mechanosensitivity of osteocytes under microgravity. Primary cilia are essential mechanosensor for osteocytes. The cilia become shorter under the simulated microgravity (SMG) environment. The cilia change may be the reason for the mechanosensitivity decrease of osteocytes under SMG. To reveal the role of primary cilia in weightless-induced osteocyte dysfunction, we investigate intraflagellar transport (IFT) to understand the mechanism of the decreased cilia length of osteocytes when subjected to SMG. We measure the number of anterograde IFT particles with GFP::IFT88 and retrograde IFT particles with OFP::IFT43 that occur at a particular transverse plane of the cilia. We also measure the expression of IFT88 and IFT43 and the size of IFT particles under SMG. Herein, the ratio of anterograde/retrograde particle number and the ratio of protein expression of IFT88/IFT43 increase under SMG. The size of anterograde IFT particles with GFP::IFT88 gets a significant decrease under SMG. Fundamentally, SMG has broken the balanced operating state of IFT and makes the IFT particles smaller. The phenomenon under SMG is intriguing. (c) 2020 Elsevier Inc. All rights reserved.