Imaging mitosis in the moss Physcomitrella patens.
Imaging mitosis in the moss Physcomitrella patens.
复制标题
对小立碗藓 (Physcomitrella patens) 苔藓的有丝分裂进行成像。
DOI：
10.1007/978-1-4939-3542-0_17
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Goshima G.
中科院分区：
文献类型：
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作者：
Yamada M;Miki T;Goshima G.
乍一看，植物的有丝分裂与动物的有丝分裂很不一样。事实上，陆生植物在进化过程中已经失去了中心体，有丝分裂纺锤体的组装独立于一个强大的微管组织中心。成膜体是植物有丝分裂后期形成的一种特殊的有丝分裂器，它向细胞皮层离心膨胀。然而，植物有丝分裂在蛋白质库水平上与动物有丝分裂的不同程度是不确定的，主要是因为植物有丝分裂基因的鉴定和体内表征的困难。在这里，我们讨论的协议，有丝分裂成像，可以结合内源性绿色荧光蛋白（GFP）标记或条件RNA干扰（RNAi）在mossPhyscomitrella小立碗藓，这是一个紧急的模式植物细胞和发育生物学。该系统有潜力用于有丝分裂和其他细胞内过程的高通量研究，如正在与各种动物细胞系。
At first glance, mitosis in plants looks quite different from that in animals. In fact, terrestrial plants have lost the centrosome during evolution, and the mitotic spindle is assembled independently of a strong microtubule organizing center. The phragmoplast is a plant-specific mitotic apparatus formed after anaphase, which expands centrifugally towards the cell cortex. However, the extent to which plant mitosis differs from that of animals at the level of the protein repertoire is uncertain, largely because of the difficulty in the identification and in vivo characterization of mitotic genes of plants. Here, we discuss protocols for mitosis imaging that can be combined with endogenous green fluorescent protein (GFP) tagging or conditional RNA interference (RNAi) in the mossPhyscomitrella patens, which is an emergent model plant for cell and developmental biology. This system has potential for use in the high-throughput study of mitosis and other intracellular processes, as is being done with various animal cell lines.