Construction of a high-density genetic linkage map and QTL mapping for sex and growth traits in Artemia franciscana
Construction of a high-density genetic linkage map and QTL mapping for sex and growth traits in Artemia franciscana
复制标题
DOI：
10.1016/j.aquaculture.2021.736692
复制
发表时间：
2021-04-09
期刊：
影响因子：
4.5
通讯作者：
Sui, Liying
中科院分区：
文献类型：
--
作者：
Han, Xuekai;Ren, Yizhuo;Sui, Liying
卤虫不仅是重要的人工活饵料，也是科学研究的理想实验生物。本研究利用SLAF-seq方法开发SNP标记，构建了首张卤虫高密度遗传连锁图谱。利用一个全同胞家系106个后代，将8709个有效SNP标记定位到21个连锁群中。该图谱的总长度为2636.43 cM，每个LG有105个位点，平均标记间距离为1.19 cM。QTL定位结果表明，在LG 6上发现了一个包含466个SNP标记的主效性QTL，其中最显著的位点可解释100%的表型变异。根据15个雌性特异SNPs的存在及其分离模式和较低的雌性/雄性重组率，进一步证实了WZ-ZZ性别决定系统在A.弗兰西斯卡纳。同时，在LG 6上的3个区间内共检测到7个与生长相关的QTL，表明LG 6可能同时参与了生长和表型性别的调节。QTL关联分析进一步确定了22个可能与生殖、生长和免疫等生物学过程相关的候选基因。本研究结果为卤虫的遗传改良和基因组研究提供了宝贵的资源。
Artemia is not only one of the most important live feed used in larviculture, but also an ideal experimental organism for scientific research. In the study, SLAF-seq method was used to develop SNP markers and construct the first high-density genetic linkage map for Artemia franciscana. A total of 8709 effective SNP markers were mapped into 21 linkage groups by using one full-sib family with 106 progenies. The map spanned 2636.43 cM and 105 loci per LG, with an average inter-marker distance of 1.19 cM. QTL mapping analysis revealed that one major sex-related QTL containing 466 sex-related SNP markers on LG6, with the most significant locus explained 100% of the phenotype variance. Given of the presence of 15 female-specific SNPs, their segregation patterns and the lower female/male recombination, the WZ-ZZ sex determining system was further confirmed in A. franciscana. Meanwhile, seven QTLs associated with growth-related traits were found within three intervals on LG6, highlighting the possible involvement of LG6 in both growth and phenotypic sex. QTL-association analysis further identified 22 candidate genes that probably related to the biological process of reproduction, growth and immunity. Our results provide a valuable resource for genetic improvement and genome study of Artemia.