Differentiation-dependent and cell-specific regulation of the hIGFBP-1 gene in human endometrium.
Differentiation-dependent and cell-specific regulation of the hIGFBP-1 gene in human endometrium.
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人子宫内膜中 hIGFBP-1 基因的分化依赖性和细胞特异性调控。
DOI：
10.1111/j.1749-6632.1997.tb48521.x
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发表时间：
1997
影响因子：
5.2
通讯作者：
Lane,B
中科院分区：
文献类型：
--
作者：
Tseng,L;Gao,J;Mazella,J;Zhu,HH;Lane,B
我们通过瞬时转染法分析了人子宫内膜间质细胞进行蜕膜化过程中IGFBP-1基因启动子的活性。一项超过13天的培养周期的时间研究表明，在用MPA和RLX处理的细胞中，启动子活性呈指数级增加到>；10(4)倍，这与内源基因的分泌速度和稳定的mRNA水平相关。缺失分析表明，IGFBP-1基因启动子中的两个区域负责IGFBP-1基因的激活。在-1到-300bp之间的基本启动子区域包含多段与Cre、Pre或CCAAT同源的功能元件。IGFBP-1基因在子宫内膜和肝组织中的激活差异主要在-2.6~-3.4kb的远端启动子区域，占总启动子活性的95%的启动子位于-3.3kb~+68kb之间。远端启动子区域的功能和结合分析表明，多个Sp1元件与一个新的SP3转录因子相互作用，激活hIGFBP-1基因启动子。
We analyzed IGFBP-1 gene promoter activity by transient transfection during the progressive decidualization of human endometrial stromal cells. A time study over a 13-day culture period showed that the promoter activity increased exponentially to> 10 (4) fold in cells treated with MPA and RLX correlating with the secretion rate and steady-state mRNA levels of the endogenous gene. Deletion analysis showed that two regions in the IGFBP-1 gene promoter are responsible for the activation of the IGFBP-1 gene. The basal promoter region between-1 and-300 bp contains multiple sections of functional elements homologous either to CRE, PRE, or CCAAT. The major difference of IGFBP-1 gene activation in endometrium and the hepatic system lies in the distal promoter region, between-2.6 and-3.4 kb, which mediates 95% of the total promoter activity derived from-3.3 kb to+ 68 bp. Functional and binding analysis in the distal promoter region showed that multiple Sp1 elements interacting with a novel Sp3 transcription factor activates the hIGFBP-1 gene promoter.