Control of floral meristem determinacy in petunia by MADS-box transcription factors
Control of floral meristem determinacy in petunia by MADS-box transcription factors
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DOI：
10.1104/pp.105.072660
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发表时间：
2006-03-01
期刊：
影响因子：
7.4
通讯作者：
Angenent, GC
中科院分区：
文献类型：
--
作者：
Ferrario, S;Shchennikova, AV;Angenent, GC
茎顶端分生组织（SAM）是一组未分化的分裂细胞，负责植物的连续生长。已经鉴定了控制SAM的发育和维持的几个基因。其中，来自拟南芥（Arabidopsis thaliana）的WUSCHEL（WUS）被认为是维持SAM中的干细胞库所必需的。MADS盒基因AGAMOUS与一个未知因子结合，被认为是WUS的负调控因子，导致花分生组织内分生组织活性的终止。产生转基因矮牵牛（Petuniahybrida）植物，其中E型和D型MADS盒基因FLORAL BindingProtein 2（FBP 2）和FBP 11分别同时过表达。这些植物在子叶阶段显示发育早期停滞。对这些转基因植物的分子分析揭示了FBP 2和FBP 11在抑制矮牵牛WUS同源物ESTATOR中可能的联合作用。此外，C型和D型同源异型基因FBP 6和FBP 7的异位上调分别表明，他们也可能参与一个复杂的，这导致转基因植物的确定性。这些数据支持了由C-、D-和E-型MADS盒蛋白组成的转录因子复合物控制花分生组织中的干细胞群体的模型。
The shoot apical meristem (SAM), a small group of undifferentiated dividing cells, is responsible for the continuous growth of plants. Several genes have been identified that control the development and maintenance of the SAM. Among these, WUSCHEL (WUS) from Arabidopsis ( Arabidopsis thaliana) is thought to be required for maintenance of a stem cell pool in the SAM. The MADS-box gene AGAMOUS, in combination with an unknown factor, has been proposed as a possible negative regulator of WUS, leading to the termination of meristematic activity within the floral meristem. Transgenic petunia ( Petunia hybrida) plants were produced in which the E-type and D-type MADS- box genes FLORAL BINDING PROTEIN2 (FBP2) and FBP11, respectively, are simultaneously overexpressed. These plants show an early arrest in development at the cotyledon stage. Molecular analysis of these transgenic plants revealed a possible combined action of FBP2 and FBP11 in repressing the petunia WUS homolog, TERMINATOR. Furthermore, the ectopic up-regulation of the C-type and D-type homeotic genes FBP6 and FBP7, respectively, suggests that they may also participate in a complex, which causes the determinacy in transgenic plants. These data support the model that a transcription factor complex consisting of C-, D-, and E-type MADS- box proteins controls the stem cell population in the floral meristem.