Shortening the Period from Crossing to the Seedling Stage through Ovule Culture of Interspecific Crosses of Azalea (Rhododendron spp.)
Shortening the Period from Crossing to the Seedling Stage through Ovule Culture of Interspecific Crosses of Azalea (Rhododendron spp.)
复制标题
杜鹃花种间杂交胚珠培养缩短杂交至苗期的时间
DOI：
10.2503/jjshs.70.54
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
I. Miyajima
中科院分区：
文献类型：
--
作者：
Ayano Michishita;K. Ureshino;I. Miyajima
为了建立杜鹃花（Rhododendronspp.）以5种1变种间双列杂交的胚珠（未成熟种子）为材料，进行离体培养。在培养基中添加50 mg·L-1的GA 3可促进胚珠萌发，最快的情况下，20个杂交组合的胚珠在杂交后3个月（3 MAC）即可萌发，但萌发率较低，不到30%。当培养4 MAC的胚珠时，从所有杂交中都获得了幼苗;超过50%的幼苗来自与R. kiusianum和R.在培养后的1和2个月内获得日本山奈作为种子亲本。结果表明，用50 mg·L ~（-1）GA_3处理4 MAC的胚珠，可早熟获得杂种苗，从杂交到苗期比用6 MAC培养的种子缩短1.5 ~ 2.5个月。
To establish a technique of obtaining azalea (Rhododendron spp.) seedlings precociously, ovules (immature seeds) from a diallel cross among 5 species and 1 variety were cultured in vitro. Adding 50 mg·liter-1 GA3 to the medium accelerated ovule germination, so that in the fastest case, 3 months after crossing (3 MAC) ovules from 20 crosses germinated, but the germination rates were low and less than 30%. When ovules 4 MAC were cultured, seedlings were obtained from all crosses ; more than 50% of seedlings from crosses with R. kiusianum and R. kaempferi as seed parents were obtained within 1 and 2 months after culturing. These results indicate that by exposing ovules of 4 MAC to 50mg·liter-1 GA3, one can obtain hybrid seedlings precociously ; the period from crossing to the seedling stage was 1.5 to 2.5 months shorter than with 6 MAC cultured seeds.