SULFOBETAINE DERIVATIVES OF BILE-ACIDS - NON-DENATURING SURFACTANTS FOR MEMBRANE BIOCHEMISTRY
SULFOBETAINE DERIVATIVES OF BILE-ACIDS - NON-DENATURING SURFACTANTS FOR MEMBRANE BIOCHEMISTRY
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DOI：
10.1016/0003-2697(83)90651-6
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
OSBORNE, JC
中科院分区：
文献类型：
--
作者：
HJELMELAND, LM;NEBERT, DW;OSBORNE, JC
本文介绍了四种新的磺基甜菜碱衍生物胆汁盐的合成，沿着提出了一套膜生物化学中有用的洗涤剂的一般标准。考察了新化合物的临界胶束浓度、聚集数、偏比容、临界胶束温度、紫外-可见光谱和圆二色谱等物理性质。为了研究这类化合物与大分子的相互作用，进一步研究了其中之一（CHAPS [3-[（3-胆酰胺丙基）二甲基铵基]-1-丙磺酸盐]）。在不同浓度的CHAPS存在下测量[人]载脂蛋白C-III 2的圆二色谱，以确定该化合物对二级结构的影响。还在CHAPS存在下进行了[小鼠肝脏]细胞色素P-450的凝胶排阻色谱和沉降平衡研究，以确定该去污剂将细胞色素P-450解聚为单体/二聚体状态的能力。
The syntheses of 4 new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, UV-visible spectrum and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compunds with macromolecules, one of these (CHAPS [3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate]) was further studied. Circular dichroism spectra of [human] apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of [mouse liver] cytochrome P-450 in the presence of CHAPS were also performed to estabilsh the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.