Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/ sulfamethoxazole.
Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/ sulfamethoxazole.
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DOI：
10.3354/dao02477
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发表时间：
2012-07
影响因子：
1.4
通讯作者：
Yuchun Liu;Zhigang Zhou;N. Wu;Y. Tao;Li Xu;Yanan Cao;Y. Zhang;B. Yao
中科院分区：
文献类型：
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作者：
Yuchun Liu;Zhigang Zhou;N. Wu;Y. Tao;Li Xu;Yanan Cao;Y. Zhang;B. Yao
甲氧苄啶/磺胺甲恶唑广泛用于治疗水产养殖中由细菌病原体引起的感染性疾病。然而，抗生素给药的做法可以促进耐药菌株的出现，并导致随着时间的推移疗效减弱。本研究的目的是评估口服甲氧苄啶/磺胺甲恶唑对健康异育银鲫和细菌性肠炎患者胃肠道（GI）微生物群的影响。利用变性梯度凝胶电泳（DGGE）技术，首次直观地描述了优势菌群的变化。结果表明：（1）放线菌门、厚壁菌门和变形菌门是异育银鲫肠道的优势菌门;（2）抗生素对异育银鲫肠道菌群的影响远大于患病鱼;（3）肠杆菌科可能是异育银鲫肠道的主要耐药菌之一。这项研究提供了一个深入了解抗生素治疗对鱼类胃肠道微生物群的建立和定殖的影响，并推测一些可能的耐药细菌。
Trimethoprim/sulfamethoxazole is widely used in the treatment of infectious diseases caused by bacterial pathogens in aquaculture. However, the practice of antibiotic administration can promote the emergence of resistant strains of bacteria and result in a wane in efficacy over time. The objective of this study was to assess the effect of oral treatment with trimethoprim/sulfamethoxazole on the gastrointestinal (GI) microbiota of healthy gibel carp and those affected with bacterial enteritis. By using denaturing gradient gel electrophoresis (DGGE), the changes in the predominant bacterial communities were directly depicted for the first time. The main findings were (1) Actinobacteria, Firmicutes and Proteobacteria were the predominant phyla in the healthy gibel carp intestine; (2) administration of antibiotics had a more profound impact on the intestinal microflora of healthy fish than of the diseased ones; and (3) Enterobacteriaceae might be one of the major drug-resistant bacteria in the gibel carp intestine. This study provides an insight into the effect of antibiotic treatment on the establishment and colonization of fish GI microbiota and speculates on some possible drug-resistant bacteria.