Solution properties of (1→3)-α-D-glucan and its sulfated derivative from Poria cocos mycelia via fermentation tank
Solution properties of (1→3)-α-D-glucan and its sulfated derivative from Poria cocos mycelia via fermentation tank
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DOI：
10.1002/bip.20332
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发表时间：
2005-09-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, L
中科院分区：
文献类型：
--
作者：
Huang, QL;Zhang, L
从通过中试规模设施发酵罐产生的茯苓菌丝体中，通过用0.5M NaOH/0.01M NaBH 4水溶液萃取分离编码为Pi-PCM 3-I的水不溶性（1 -> 3）-α-（D）-葡聚糖。通过非溶剂添加法从Pi-PCM 3-I样品中分离出重均分子量（M）范围为7.75 × 10（4）至57.3 × 10（4）的F1至F9九个级分。将这些级分与氯磺酸-吡啶络合物反应以产生编码为S1至S8的水溶性硫酸化衍生物，其中M-w为2.36 × 104（2）至14.5 × 104（112），取代度（DS）为0.86-1.38。通过激光散射（LLS）、与LLS结合的尺寸排阻色谱法（SEC-LLS）和25 ℃下的粘度计测量天然和硫酸化Pi-PCM 3-I的M-w，z-平均回转半径（< s（2）> z（1/2））、第二维里系数（AA）和特性粘度（[77]）。Pi-PCM 3-I在0.25 M LiCl/二甲亚砜（DMSO）（Me 2SO）中的Mark-Houwink方程及其硫酸化衍生物在0.15 M NaCl水溶液中在25 ℃下的Mark-Houwink方程确定为[eta] = 1.33 x 10（-2）M-w（0.75 +/- 0.01）（mL g（-1））和[eta] = 1.46 x 10（-4）M-w（1.13 +/- 0.01）（mL g（-1））。基于蠕虫状圆柱体模型的理论，天然和硫酸化Pi-PCM 3-I的构象参数计算为每单位轮廓长度（M-L）的摩尔质量为760 +/- 50和1060 +/- 30 nm（-1），持久长度（q）为6.3 +/- 0.5和13.1 +/- 1 nm，特性比（C正比于）分别为14.9 +/- 0.2和31.8 +/- 1。结果表明，Pi-PCM 34在0.25 M LiCl/Me 2SO中以伸展的柔性链存在，其硫酸化衍生物在0.15 M NaCl水溶液中以半刚性链存在。此外，Pi-PCM 34具有类似的结构和分子参数的wc-PCM 3-I从旋转振荡器，这表明有前途的工业化的茯苓多糖。(c)2005 Wiley Periodicals，Inc.
From Poria cocos mycelia yielded via a pilot scale facility-fermentation tank, a water insoluble (1 -> 3)-alpha-(D)-glucan coded as Pi-PCM3-I was isolated by extraction with 0.5 M NaOH/ 0.01 M NaBH4 aqueous solution. Nine fractions from F1 to F9 with a weight-average molecular mass (M,) range from 7.75 x 10(4) to 57.3 x 10(4) were preparedfrom the Pi-PCM3-I sample by a nonsolvent addition method. The fractions were reacted with chlorosulfonic acid-pyridine complex to product water-soluble sulfated derivatives coded as S1 to S8 with M-w from 2.36 x 10(4) to 14.5 x 104 2)112), and degree of substitution (DS) of 0.86-1.38. M-w, z-average radius of gyration (< s(2)> z(1/2)) the second virial coefficient (AA and the intrinsic viscosity ([77]) of the native and sulfated Pi-PCM3-I were measured by laser light scattering (LLS), size-exclusion chromatography combined with LLS (SEC-LLS), and viscometry at 25 degrees C. The Mark-Houwink equations for Pi-PCM3-I in 0.25 M LiCl/ dimethylsulfoxide (DMSO) (Me2SO) and for its sulfated derivative in 0.15 M NaCl aqueous solution at 25 degrees C were established to be [eta] = 1.33 x 10(-2) M-w(0.75 +/- 0.01) (mL g(-1)) and [eta] = 1.46 x 10(-4) M-w(1.13 +/- 0.01) (mL g(-1)), respectively. On the basis of theories for a wormlike cylinder model, the conformational parameters of the native and sulfated Pi-PCM3-I were calculated to be 760 +/- 50 and 1060 +/- 30 nm(-1) for the molar mass per unit contour length (M-L), 6.3 +/- 0.5 and 13.1 +/- 1 nm for the persistence length (q), and 14.9 +/- 0.2 and 31.8 +/- 1 for the characteristic ratio (C proportional to), respectively. The results revealed that Pi-PCM34 existed as an extended flexible chain in 0.25 M LiCl/Me2SO, and its sulfated derivative existed as a semistiff chain in 0.15 M NaCl aqueous solution. Furthermore, Pi-PCM34 possessed similar structure and molecular parameters to wc-PCM3-I from a rotary shaker; this suggests promising industrialization of Poria cocos polysaccharides. (c) 2005 Wiley Periodicals, Inc.