Wide-Compatibility Gene S5n Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids
Wide-Compatibility Gene S5n Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids
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DOI：
10.2135/cropsci2011.04.0232
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发表时间：
2012-03-01
期刊：
影响因子：
2.3
通讯作者：
Lu Yong-Gen
中科院分区：
文献类型：
--
作者：
Yang You-Xin;Li Yan-Hong;Lu Yong-Gen
Embryo-sac abortion is one of the most important reasons for hybrid sterility between indica and japonica subspecies. Wide compatibility gene S-5(n) can overcome the embryo-sac sterility between indica-japonica hybrids caused by the S-5 locus located on chromosome 6. In this paper, key sequence analysis and functional molecular markers were used to identify S-5(n) gene-harboring genotypes from 646 rice (Oryza sativa L.) genotypes, including 323 cultivated and 323 wild rice genotypes. Whole-mount eosin B-staining confocal laser scanning microscopy was employed to observe the embryo-sac fertility of hybrids. A total of 28 genotypes, including 16 cultivated, 9 Oryza rufipogon accessions, and 3 Oryza nivara accessions, were found to have the S-5(n) gene. These results suggested that the S-5(n) gene differentiated and evolved from wild rice. Different indica-japonica hybrids were developed using O. sativa genotypes carrying the S-5(n) gene and without the S-5(n) gene. Embryosac fertility was more than 93% in the S-5(n) gene-harboring hybrids, whereas embryo-sac fertility was relatively low in control hybrids between typical indica and japonica cultivars without the S-5(n) gene, suggesting that S-5(n) can overcome the sterility between indica-japonica hybrids. Furthermore, it verified the reliability of molecular functional markers to detect S5n gene-harboring genotypes. Functional molecular markers used in the present study will be very useful to identify S-5(n) gene-harboring genotypes, and genotypes detected with the S-5(n) gene in the present study are very important germplasm for overcoming intersubspecific hybrid sterility.
