Heme-heme orientation and electron transfer kinetic behavior of multisite oxidation-reduction enzymes.
Heme-heme orientation and electron transfer kinetic behavior of multisite oxidation-reduction enzymes.
复制标题
多位点氧化还原酶的血红素-血红素取向和电子转移动力学行为。
DOI：
10.1126/science.6415814
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Gray,HB
中科院分区：
文献类型：
--
作者：
Makinen,MW;Schichman,SA;Hill,SC;Gray,HB
铜绿假单胞菌细胞色素cd1的偏振单晶吸收光谱分析表明，每个亚基中的血红素c和血红素d1基团在酶的氧化和还原形式中都彼此垂直取向。这些结果与之前的动力学研究结果一起表明，垂直的血红素-血红素方向可能是指定一系列连续电子转移反应中动力学缓慢步骤的重要因素。
Analysis of the polarized single-crystal absorption spectra of cytochrome cd1ofPseudomonas aeruginosashows that the heme c and heme d1groups in each subunit are oriented perpendicularly to each other in both oxidized and reduced forms of the enzyme. These results, together with those of previous kinetic studies, indicate that a perpendicular heme-heme orientation may be an important factor in specifying kinetically slow steps in a sequential series of electron transfer reactions.