Assessment of Ozone-Induced Lung Injury in Mice.
Assessment of Ozone-Induced Lung Injury in Mice.
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DOI：
10.1007/978-1-4939-8570-8_19
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Garantziotis S
中科院分区：
文献类型：
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作者：
Stober VP;Garantziotis S
臭氧是我们呼吸的空气中的一种主要污染物，臭氧水平升高会导致严重的发病率和死亡率。随着气候变暖，臭氧水平预计会增加。因此，评估臭氧引起的肺部疾病的机制的研究至关重要。本章描述了臭氧暴露的小鼠模型和评估臭氧对肺部影响的方法。包括支气管肺泡灌洗、尸检和肺功能测量。灌洗可评估空气中的细胞渗透、细胞因子产生、组织损伤和毛细血管渗漏。尸检为全肺的基因表达、组织学和蛋白质评估提供了组织。肺生理学用于评估呼吸道高反应性、组织和总肺阻力、顺应性和弹性。
Ozone is a major pollutant in the air we breathe, and elevated levels lead to significant morbidity and mortality. As the climate warms, levels of ozone are predicted to increase. Accordingly, studies to assess the mechanisms of ozone-induced lung diseases are paramount. This chapter describes mouse models of ozone exposure and methods for assessing the effects of ozone in the lungs. These include bronchoalveolar lavage, necropsy, and measurement of lung function. Lavage allows for assessment of cell infiltration, cytokine production, tissue damage and capillary leakage in the airspaces. Necropsy provides tissue for gene expression, histology, and protein assessment in the whole lung. Lung physiology is used to assess airway hyperresponsiveness, tissue and total lung resistance, compliance, and elastance.