Microtechnique of sperm immobilization test.
Microtechnique of sperm immobilization test.
复制标题
精子固定试验的显微技术。
DOI：
10.1016/s0015-0282(16)57550-5
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发表时间：
1978
影响因子：
6.7
通讯作者：
K. Koyama
中科院分区：
文献类型：
--
作者：
S. Isojima;K. Koyama
3个表格构成了本报告的核心，它描述了一种确定宫颈粘液中是否存在精子固定因子的微技术。表1是显示原始试管法精子固定测试程序的流程图；表2显示了使用一次性微室检查宫颈粘液小样本中抗体的微精子固定测试程序；表3描述了精子制备。另外两个表总结了精子固定试验的结果：表4给出了15名患者在试管和微量两种方法下1、2和3小时的结果，用于比较；表5显示了使用血清中有精子固定抗体的妇女的宫颈粘液时的微精子固定试验结果。结果表明，2/3的宫颈粘液标本具有精子固定因子。血清中没有精子固定抗体的不孕症妇女宫颈粘液中精子固定值为阴性。血清中无精子固定抗体的不孕症妇女宫颈粘液中精子固定值的阳性结果强烈表明，宫颈内精子的局部抗体产生是可能的。用较少的宫颈粘液标本和灭活补体代替试管法中使用的对照阴性血清来证明精子固定抗体的微量，两种滴度，即原始试管和新微量滴度均显示平行值。
3 tables form the core of this presentation which describes a microtechnique for determining the presence of sperm-immobilizing factor in cervical mucus. Table 1 is a flow chart showing the procedure of the original tube method of sperm immobilization testing; table 2 shows procedures of the microsperm immobilization test which uses a disposable microchamber to examine antibody in small specimens of cervical mucus; table 3 describes the sperm preparation. 2 other tables summarize results of sperm immobilizing tests: table 4 gives 1 2 and 3 hour results for 15 patients of both the tube and micro method for comparison; table 5 presents results of the microsperm immobilizing test when using cervical mucus from women with demonstrated sperm-immobilizing antibody in serum. It was clearly shown that 2/3 cervical mucus samples possessed the sperm-immobilizing factor. Sperm immobilization values in cervical mucus from sterile women who did not have the sperm-immobilizing antibody in their serum showed negative sperm immobilization values in the cervical mucus. The positive sperm immobilization values in cervical mucus from sterile women who did not have sperm-immobilizing antibody in their serum showed results that strongly suggested that local antibody production to spermatozoa in the cervical canal could be possible. The microtechnique to prove the sperm-immobilizing antibody uses a smaller amount of cervical mucus specimen and inactivated complement instead of control negative serum used in the tube method; both titers i.e. original tube and new micro showed parallel values.