High-Resolution, Two-Dimensional Measurement of Dissolved Reactive Phosphorus in Sediments Using the Diffusive Gradients in Thin Films Technique in Combination with a Routine Procedure
High-Resolution, Two-Dimensional Measurement of Dissolved Reactive Phosphorus in Sediments Using the Diffusive Gradients in Thin Films Technique in Combination with a Routine Procedure
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使用薄膜扩散梯度技术与常规程序相结合，对沉积物中溶解的活性磷进行高分辨率、二维测量
DOI：
10.1021/es202785p
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发表时间：
2011-11-15
影响因子：
11.4
通讯作者：
Zhang, Chaosheng
中科院分区：
文献类型：
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作者：
Ding, Shiming;Jia, Fei;Zhang, Chaosheng
溶解活性磷（DRP）是沉积物中最有效的磷形态，常常直接控制水生系统中的浮游植物水华。在这项研究中，开发了一种新的程序二维（2D）测量沉积物中的DRP在0.45 mm的空间分辨率使用扩散梯度薄膜（DGT）技术与修改后的高容量结合相（锆氧化物凝胶）。该程序包括沉积物中P的DGT摄取，在0.45 x 0.45 mm网格系统上结合凝胶的2D切片，用1 M NaOH从每个凝胶正方形中洗脱P，并使用384微孔板对每个洗脱溶液中的DRP进行微量比色测定。通过这一程序对DRP的测量在均匀溶液和沉积物中进行了测试，并产生了可接受的误差（
Dissolved reactive phosphorus (DRP) is the most available P form in sediments and often directly controls phytoplankton blooms in aquatic systems. In this study, a novel procedure was developed for two-dimensional (2D) measurement of DRP in sediments at a spatial resolution of 0.45 mm using the diffusive gradients in thin films (DGT) technique with a revised high-capacity binding phase (Zr oxide gel). This procedure involves DGT uptake of P in sediments, 2D slicing of the binding gel on a 0.45 x 0.45-mm grid system, elution of P from each gel square with 1 MNaOH, and microcolorimetric determination of DRP in each eluted solution using 384-microwell plates. Measurements of DRP via this procedure were tested in homogeneous solutions and sediments and produced an acceptable error (