Domain-Specific Regulation of Recombination in Caenorhabditis elegans in Response to Temperature, Age and Sex
Domain-Specific Regulation of Recombination in Caenorhabditis elegans in Response to Temperature, Age and Sex
复制标题
DOI：
10.1534/genetics.108.090142
复制
发表时间：
2008-10-01
期刊：
影响因子：
3.3
通讯作者：
Yanowitz, Judith L.
中科院分区：
文献类型：
--
作者：
Lim, Jaclyn G. Y.;Stine, Rachel R. W.;Yanowitz, Judith L.
一般认为，减数分裂重组率随着温度的升高而增加，随着年龄的增加而降低，并且存在性别差异。我们使用包含第三号染色体96%的物理标记，重新研究了这些因素对线虫减数分裂重组的影响。在卵母细胞和雄性精子之间唯一的总体交换频率的差异是在16度。此外，不同生殖系之间的交叉干扰(CI)也不同，卵母细胞表现出比雄性精子更高的交叉干扰。出乎意料的是，我们的分析显示，两性卵母细胞的交叉分布随着温度的变化而发生显著变化。这一特征似乎是线虫染色体的一般特征，因为X染色体也有类似的温度反应变化。我们还发现，在两性和雌性中，杂交的分布都随着年龄的变化而变化。我们的观察表明，来自最年轻母亲的卵母细胞--而不是年龄最大的母亲--表现出了不同的交叉模式。我们的数据支持了一个新的假设，即线虫和人类一样，重组是在大的染色体域中受到调控的。
It is generally considered that meiotic recombination rates increase with temperature, decrease with age, and differ between the sexes. We have reexamined the effects of these factors on meiotic recombination in the nematode Caenorhabditis elegans using physical markers that encompass >96% of chromosome III. The only difference in overall crossover frequency between oocytes and male sperm was observed at 16 degrees. In addition, crossover interference (CI) differs between the germ lines, with oocytes displaying higher CI than male sperm. Unexpectedly, our analyses reveal significant changes in crossover distribution in the hermaphrodite oocyte in response to temperature. This feature appears to be a general feature of C. elegans chromosomes as similar changes in response to temperature are seen for the X chromosome. We also find that the distribution of crossovers changes with age in both hermaphrodites and females. Our observations indicate that it is the oocytes from the Youngest mothers-and not the oldest-that showed a different pattern of crossovers. Our data enhance the emerging hypothesis that recombination in C. elegans, as in humans, is regulated in large chromosomal domains.