The bone lining cell:: Its role in cleaning Howship's lacunae and initiating bone formation
The bone lining cell:: Its role in cleaning Howship's lacunae and initiating bone formation
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DOI：
10.1359/jbmr.2002.17.1.77
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发表时间：
2002-01-01
影响因子：
6.2
通讯作者：
Beertsen, W
中科院分区：
文献类型：
--
作者：
Everts, V;Delaissé, JM;Beertsen, W
在这项研究中，我们研究了骨衬里细胞在骨吸收和骨形成的协调中的作用。小鼠长骨和颅骨的超微结构分析显示，骨衬里细胞包裹并随后消化从破骨细胞留下的Howship陷窝突出的胶原纤维。通过使用选择性蛋白酶抑制剂，我们表明，这种消化依赖于基质金属蛋白酶，并在一定程度上，丝氨酸蛋白酶。放射自显影显示，在骨衬细胞完成清洁后，它们存款一薄层胶原基质沿着Howship的陷窝，与富含骨桥蛋白的骨水泥线密切相关。仅在完全清洁的窝中检测到胶原基质沉积。在骨密度不全患者和组织蛋白酶K缺陷小鼠的骨中，骨细胞骨基质消化受到极大抑制的条件下，骨基质残留物被骨衬里细胞降解，从而表明骨衬里细胞“拯救”了这些异常中的骨重建。我们的结论是，去除骨胶原的破骨细胞在Howship的陷窝是骨吸收和形成之间的联系，骨衬里细胞和基质金属蛋白酶是必不可少的在这个过程中的一个必要步骤。
In this study we investigated the role of bone lining cells in the coordination of bone resorption and formation. Ultrastructural analysis of mouse long bones and calvariae revealed that bone lining cells enwrap and subsequently digest collagen fibrils protruding from Howship's lacunae that are left by osteoclasts. By using selective proteinase inhibitors we show that this digestion depends on matrix metalloproteinases and, to some extent, on serine proteinases. Autoradiography revealed that after the bone lining cells have finished cleaning, they deposit a thin layer of a collagenous matrix along the Howship's lacuna, in close association with an osteopontin-rich cement line. Collagenous matrix deposition was detected only in completely cleaned pits. In bone from pycnodysostotic patients and cathepsin K-deficient mice, conditions in which osteoclastic bone matrix digestion is greatly inhibited, bone matrix leftovers proved to be degraded by bone lining cells, thus indicating that the bone lining cell "rescues" bone remodeling in these anomalies. We conclude that removal of bone collagen left by osteoclasts in Howship's lacunae is an obligatory step in the link between bone resorption and formation, and that bone lining cells and matrix metalloproteinases are essential in this process.