A Bacterial Isolate Capable of Quenching Both Diffusible Signal Factor- and N-Acylhomoserine Lactone-Family Quorum Sensing Signals Shows Much Enhanced Biocontrol Potencies.
A Bacterial Isolate Capable of Quenching Both Diffusible Signal Factor- and N-Acylhomoserine Lactone-Family Quorum Sensing Signals Shows Much Enhanced Biocontrol Potencies.
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能够猝灭扩散信号因子和 N-酰基高丝氨酸内酯家族群体感应信号的细菌分离物显示出大大增强的生物防治效力
DOI：
10.1021/acs.jafc.2c01299
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发表时间：
2022-06-29
影响因子：
6.1
通讯作者：
Zhang, Lian-Hui
中科院分区：
文献类型：
--
作者：
Wang, Huishan;Lin, Qiqi;Dong, Lingling;Wu, Wenting;Liang, Zhibing;Dong, Zhangyong;Ye, Huijuan;Liao, Lisheng;Zhang, Lian-Hui
N-酰基高丝氨酸内酯(AHL)和扩散信号因子(DSF)分子是两类广泛保守的群体感应(QS)信号。通过酶法灭活QS信号的群体猝灭(QQ)是一种很有前途的生防策略。在寻找既能猝灭AHL信号又能猝灭DSF信号的生防剂的过程中，最近发现了DSF猝灭生防剂假单胞菌。HS-18包含至少三个编码AHL-酰基酶的基因(aigA、aigB和aigC)，在不同的AHL上表现出很强的AHL-酰基酶活性。其中，AIGA和AigC对AHLS表现出广谱酶活性，而AigB偏好较长的AHLS。有趣的是，aigC的转录表达可被AHL信号显著诱导。异源表达AIGA-C的铜绿假单胞菌和阴沟伯克霍尔德氏菌的AHL积累、毒力因子产生、生物膜形成、运动性和对植物的毒力显著降低。值得注意的是，HS-18中的两种类型的QQ机制在增强对AHL和DSF依赖病原菌的生防效力方面显示出强烈的和非常理想的协同效应。
N-Acylhomoserine lactone (AHL) and diffusible signal factor (DSF) molecules are two families of widely conserved quorum sensing (QS) signals. Quorum quenching (QQ) via enzymatic inactivation of QS signals is a promising strategy of biocontrol. In the search for biocontrol agent quenching both AHL and DSF signals, it has been recently identified that DSF-quenching biocontrol agent Pseudomonas sp. HS-18 contains at least three genes (aigA, aigB, and aigC) encoding AHL-acylases displaying strong AHL-acylase activities on various AHLs. Among them, AigA and AigC presented broad-spectrum enzyme activity against AHLs, while AigB preferred longer AHLs. Interestingly, transcriptional expression of aigC could be significantly induced by AHL signals. Heterologous expression of aigA–C in Burkholderia cenocepacia and Pseudomonas aeruginosa resulted in drastically decreased AHL accumulation, virulence factor production, biofilm formation, motility, and virulence on plants. Significantly, the two types of QQ mechanisms in HS-18 showed a strong and much desired synergistic effect for enhanced biocontrol potency against AHL- and DSF-dependent pathogens.
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影响因子：
3.6
作者：
Easlon HM;Bloom AJ
通讯作者：
Bloom AJ
顺式-2-十二碳烯酸信号的结构类似物破坏新洋葱伯克霍尔德菌的群体感应和毒力
影响因子：
4.4
作者：
Cui, Chaoyu;Song, Shihao;Deng, Yinyue
通讯作者：
Deng, Yinyue
影响因子：
4.4
作者：
Huang, JJ;Han, JI;Leadbetter, JR
通讯作者：
Leadbetter, JR
影响因子：
3.6
作者：
Lin, YH;Xu, JL;Zhang, LH
通讯作者：
Zhang, LH
DOI：
10.1099/00221287-143-12-3703
发表时间：
1997-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
McClean, KH;Winson, MK;Williams, P
通讯作者：
Williams, P