cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA.
cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA.
复制标题
大鼠前内皮素原-1 mRNA 的 cDNA 克隆、序列分析和组织分布。
DOI：
10.1016/s0006-291x(05)81197-0
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Tomoh Masaki
中科院分区：
文献类型：
--
作者：
Takeshi Sakurai;M. Yanagisawa;Akihiro Inoue;Una S. Ryan;Sadao Kimura;Youji Mitsui;K. Goto;Tomoh Masaki
我们报告的全长cDNA编码大鼠preproET-1（preproET-1）的克隆。预测的大鼠preproET-1由202个氨基酸残基组成，分别与人、猪和牛的preproET-1高度相似。成熟大鼠ET-1的21个氨基酸残基序列与人、猪、犬和牛ET-1完全相同。与其他哺乳动物物种一样，预测大鼠中的成熟ET-1由39个残基的大ET-1产生。北方印迹分析表明，一个单一的2.3 kb的preproET-1 mRNA不仅表达在血管内皮细胞，而且在其他大鼠组织，包括肺，脑，子宫，胃，心脏，肾上腺和肾脏。提示ET-1可能在大鼠心血管系统内外多器官中发挥局部介质的作用。
We report the cloning of a full-length cDNA encoding rat preproendothelin-1 (preproET-1). The predicted rat preproET-1 consists of 202 amino acid residues and highly similar to human, porcine and bovine preproET-1, respectively. The deduced 21-residue sequence of mature rat ET-1 is identical to human, porcine, canine and bovine ET-1. As in other mammalian species, the mature ET-1 is predicted to be produced from a 39-residue big ET-1 in the rat. Northern blot analysis showed that a single 2.3-kb preproET-1 mRNA is expressed not only in vascular endothelial cells but also in other rat tissues, including the lung, brain, uterus, stomach, heart, adrenal gland and kidney. These findings suggest that ET-1 may play roles as a local mediator in multiple organs both within and outside the cardiovascular system in the rat.