HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins
HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins
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DOI：
10.1093/emboj/19.13.3377
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发表时间：
2000-07-03
期刊：
影响因子：
11.4
通讯作者：
Varga-Weisz, PD
中科院分区：
文献类型：
--
作者：
Poot, RA;Dellaire, G;Varga-Weisz, PD
含有核小体依赖的ATP酶ISWI的染色质重塑复合物首先从果蝇胚胎中分离得到（BMF、CHRAC和ACF），ISWI是这些复合物中唯一的共同组分。我们对人CHRAC（HuCHRAC）的纯化表明，ISWI染色质重塑复合物在完全不同的细胞类型中可以具有保守的亚基组成，表明ISWI的保守功能。我们表明，果蝇CHRAC中两种新的推定组蛋白折叠蛋白的人类同源物存在于HuCHRAC中，这两种人类组蛋白折叠蛋白形成稳定的复合物，其结合裸DNA而不结合核小体，HuCHRAC还含有人ACF 1（hACF 1），其是果蝇ACF的亚基Acf 1的同源物，小鼠ACF 1的N-末端被报道为异染色质靶向结构域，hACF 1是具有相关结构域结构的蛋白质家族的成员，其都可以靶向异染色质。我们讨论了一个可能的功能HuCHRAC异染色质动力学。HuCHRAC不含拓扑异构酶IT，其早先被报道为果蝇CHRAC的亚基。
Chromatin remodelling complexes containing the nucleosome-dependent ATPase ISWI were first isolated from Drosophila embryos (NURF, CHRAC and ACF), ISWI was the only common component reported in these complexes. Our purification of human CHRAC (HuCHRAC) shows that ISWI chromatin remodelling complexes can have a conserved subunit composition in completely different cell types, suggesting a conserved function of ISWI, We show that the human homologues of two novel putative histone-fold proteins in Drosophila CHRAC are present ill HuCHRAC, The two human histone-fold proteins form a stable complex that binds naked DNA but not nucleosomes, HuCHRAC also contains human ACF1 (hACF1), the homologue of Acf1, a subunit of Drosophila ACF, The N-terminus of mouse ACF1 was reported as a heterochromatin-targeting domain, hACF1 is a member of a family of proteins with a related domain structure that all may target heterochromatin. We discuss a possible function for HuCHRAC in heterochromatin dynamics. HuCHRAC does not contain topoisomerase IT, which was reported earlier as a subunit of Drosophila CHRAC.