Characterization of post-mating interspecific cross-compatibility in Prunus (Rosaceae)
Characterization of post-mating interspecific cross-compatibility in Prunus (Rosaceae)
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DOI：
10.1016/j.scienta.2018.11.045
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发表时间：
2019-02-27
影响因子：
4.3
通讯作者：
Tao, Ryutaro
中科院分区：
文献类型：
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作者：
Morimoto, Takuya;Kitamura, Yuto;Tao, Ryutaro
种间杂交常常受到从早期授粉到后代的初始生长和生殖阶段的杂交的各个阶段发生的不亲和反应的阻碍。生殖障碍的表征导致进一步了解每个物种如何识别自己的身份，并支持开发克服障碍和产生种间杂交种的方法。研究了杏、鸟李、梅、桃、柳5种李属植物的种间杂交亲和性。活体花粉管生长试验表明，许多杂交组合在交配后存在合子前障碍。无论杂交方向如何，在与鸟肺孢子虫的种间杂交中观察到花粉管生长减少，鸟肺孢子虫是一种遗传上远离其他物种的物种。在某些种间杂交中，正反交的亲和性反应不同。桃作为花粉供体与梅、柳亲和，正反交不亲和。此外，柳杨作为雌蕊亲本接受杏、梅和桃的花粉，而柳杨与这些种的正反交不亲和。这些结果表明，种间杂交（不）亲和性不仅涉及系统发育关系，还可能涉及其他机制。胚拯救培养表明，在梅作为种子亲本与桃作为花粉亲本的杂交中存在合子后障碍。讨论了影响种间杂交的因素，特别是亲本间的遗传距离和雌蕊长等形态性状。
Interspecific hybridization is often hindered by incompatibility reactions occurring at various stages of hybridization, from early pollination to initial growth and reproductive stages of the progeny. Characterization of reproductive barriers leads to further understanding of how each species recognizes its own identity and supports the development of methods to overcome the barrier and produce interspecific hybrids. We investigated interspecific cross-(in)compatibility among five species in Prunus (Rosaceae), namely P. armeniaca, P. avium, P. mume, P. persica and P. salicina. In vivo pollen tube growth tests showed the presence of a post-mating pre-zygotic barrier in many cross combinations. Irrespective of the crossing direction, reduced pollen tube growth was observed in interspecific hybridization with P. avium, a species phylogenetically remote from the other species. Different compatibility reactions in reciprocal crosses were observed in some interspecific hybridizations. P. persica as a pollen donor was compatible with P. mume and P. salicina, while the reciprocal cross was incompatible. Furthermore, P. salicina as a pistil parent accepted pollen from P. armeniaca, P. mume and P. persica, while P. salicina was cross-incompatible with those species in reciprocal crosses. These results suggest that not only the phylogenetic relationship but also other mechanisms may be involved in interspecific cross-(in)compatibility. Embryo rescue culture showed the presence of a post-zygotic barrier in the cross between P. mume as seed parent and P. persica as pollen parent. Factors affecting interspecific hybridization are discussed with special reference to genetic distance between parents and morphological characters such as pistil length.