Subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase show differential and distinct expression patterns during germ cell differentiation: alternative polyadenylation in germ cells gives rise to unique smaller-sized mRNA species.
Subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase show differential and distinct expression patterns during germ cell differentiation: alternative polyadenylation in germ cells gives rise to unique smaller-sized mRNA species.
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环腺苷 3,5-单磷酸依赖性蛋白激酶的亚基在生殖细胞分化过程中表现出差异化和独特的表达模式：生殖细胞中的替代多腺苷酸化产生独特的较小尺寸的 mRNA 种类。
DOI：
10.1095/biolreprod43.1.46
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发表时间：
1990
影响因子：
3.6
通讯作者：
T. Jahnsen
中科院分区：
文献类型：
--
作者：
O. Øyen;F. Myklebust;John D. Scotf;G. Cadd;G. Stanley Mcknight;Vidar Hansson;T. Jahnsen
CAMP(CAMP)和cAMP依赖的蛋白激酶(PKA)被认为参与调节精子的基本功能，如运动、附睾成熟、获能和顶体反应。在这项研究中，我们记录了生殖细胞中5个不同的PKA亚基(RIα、RIβ、RIIα、RIIβ和Cα)的显著mRNA水平，并展示了这些亚基在精子发生过程中的差异表达模式。RI(RIα和RIβ)和Cα的信使RNA似乎在减数分裂前生殖细胞阶段被诱导，而RII(RIIα和RIIβ)的mRNAs首先在单倍体阶段表达。单独的PKA亚基可能在生殖细胞和成熟精子的发育中传递特定的功能。本研究进一步证明，与体细胞相比，生殖细胞中存在独特的较小尺寸的mRNAs。在生殖细胞中似乎选择了特定的截短形式的RIα、RIIα、RIIβ和CαmRNAs。我们的数据表明这是由于使用了替代的多聚腺苷酸化位点信号。选择较短的、稳定性较高的信使核糖核酸可能是精子细胞中观察到的延迟翻译所必需的。这可能确保特定水平的信使核糖核酸在精子细胞晚期转录停止后进行翻译。
Cyclic AMP (cAMP) and cAMP-dependent protein kinases (PKAs) are believed to be involved in the regulation of essential spermatozoal functions, such as motility, epididymal maturation, capacitation, and the acrosome reaction. In this study, we document the presence of significant mRNA levels for 5 different PKA subunits (RI alpha, RI beta, RII alpha, RII beta, and C alpha) in germ cells and demonstrate differential expression patterns for these subunits during spermatogenesis. Messenger RNAs for RI (RI alpha and RI beta) and C alpha appear to be induced at premeiotic germ cell stages, whereas mRNAs for RII (RII alpha and RII beta) are first expressed at haploid stages. The individual PKA subunits may convey specific functions in developing germ cells and mature sperm. The present study, furthermore, demonstrates the presence of unique smaller-sized mRNAs in germ cells compared with somatic cells. Specific, truncated forms of RI alpha, RII alpha, RII beta, and C alpha mRNAs appear to be selected in the germ cells. Our data suggest this to be due to the use of alternative polyadenylation site signals. The selection of shorter mRNA species, with higher stability, may be essential for the delayed translation observed in spermatids. This may ensure certain levels of mRNA for translation at late spermatid stages, after cessation of transcription.