Role of physicochemical properties of coating ligands in receptor-mediated endocytosis of nanoparticles
Role of physicochemical properties of coating ligands in receptor-mediated endocytosis of nanoparticles
复制标题
涂层配体的理化性质在受体介导的纳米颗粒内吞作用中的作用
DOI：
10.1016/j.biomaterials.2012.04.055
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发表时间：
2012-08-01
期刊：
影响因子：
14
通讯作者：
Ma, Yu-qiang
中科院分区：
文献类型：
--
作者：
Ding, Hong-ming;Ma, Yu-qiang
对受体介导的内吞作用的基本认识在生物医学中具有重要意义。本文利用耗散粒子动力学方法系统研究了包被配体的性质对细胞摄取纳米粒子的影响，发现受体-配体相互作用的强度、配体的密度、长度以及刚性都能强烈影响受体介导的内吞作用的最终平衡。有趣的是，发现配体越长，修饰的粒子越容易附着在膜上，而越难被完全吞噬。增加配体的密度和刚性，增强配体在颗粒上的均匀分布，可能导致总吞没。此外，我们还表明，如果可以合理地设计配体的疏水/疏脂性能的颗粒可以被完全吞没。目前的研究表明，不仅配体的化学参数，而且配体的物理参数都可以决定纳米颗粒与细胞的相互作用，这可能会为未来药物输送中的纳米颗粒设计提供一些重要的见解。(C)2012爱思唯尔有限公司保留所有权利。
A fundamental understanding of the receptor-mediated endocytosis is of great importance in biomedicine. In this paper, we systematically investigate the effect of the properties of coating ligands on the cellular uptake of nanoparticles by using dissipative particle dynamics, and find that the strength of the receptor-ligand interaction, the ligand density and length as well as its rigidity can strongly affect the final equilibrium in the receptor-mediated endocytosis. Interestingly, it is found that the particle decorated with longer ligands is more likely to attach to the membrane, while it is harder to be totally engulfed. Increasing the ligand density and rigidity which enhances the uniform distribution of ligands on the particle may lead to the total engulfment. Further, we also show that the particle can be totally engulfed if one can reasonably design the hydrophobic/lipophobic properties of ligands. The present study shows that not only the chemical but also the physical parameters of ligands can govern the nanoparticle-cell interaction, which may give some significant insights into future nanoparticle design in drug delivery. (C) 2012 Elsevier Ltd. All rights reserved.