Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand.
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand.
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DOI：
10.1186/s12936-017-1826-8
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发表时间：
2017-04-28
期刊：
影响因子：
3
通讯作者：
Mueller I
中科院分区：
文献类型：
--
作者：
Longley RJ;França CT;White MT;Kumpitak C;Sa-Angchai P;Gruszczyk J;Hostetler JB;Yadava A;King CL;Fairhurst RM;Rayner JC;Tham WH;Nguitragool W;Sattabongkot J;Mueller I
泰国的目标是到2024年消除疟疾。间日疟原虫现已成为该国导致疟疾的主要物种，并且很高比例的感染没有症状。在获得性免疫应答特征不明显的低传播环境中，更好地了解间日疟原虫抗原的抗体动力学对于开发新的消除策略（如改进的监测方法和疫苗）至关重要。本研究的目的是表征泰国西部一个村庄11种关键间日疟原虫蛋白的总IgG抗体水平。使用了2012年在坎恰纳布里进行的横断面调查中招募的546名志愿者的血浆样本。使用基于微珠的多重测定法测量已知或预测参与网织红细胞结合或侵袭的11种不同蛋白（阿普、GAMA、P41、P12、PVX_081550和PvRBP家族的5个成员）以及主要红细胞前候选疫苗（CSP）的总IgG水平。探讨了IgG水平与感染状态、年龄和空间位置之间的关系。来自泰国西部低传播地区的个体对所有11种间日疟原虫重组蛋白都有反应。尽管所有感染个体均无症状，但在存在当前间日疟原虫感染的情况下观察到显著更高的IgG水平。成人（18岁及以上）的IgG水平也高于儿童。对于大多数蛋白质，在靠近缅甸边境和远离当地卫生服务的个人中观察到较高的IgG水平。观察到对大多数蛋白质和与暴露替代物相关的IgG水平的稳健IgG应答，表明这些抗原可作为暴露、免疫或两者的潜在生物标志物。本文的在线版本（doi：10.1186/s12936-017-1826-8）包含补充材料，可供授权用户使用。
Thailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become the dominant species causing malaria within the country, and a high proportion of infections are asymptomatic. A better understanding of antibody dynamics to P. vivax antigens in a low-transmission setting, where acquired immune responses are poorly characterized, will be pivotal for developing new strategies for elimination, such as improved surveillance methods and vaccines. The objective of this study was to characterize total IgG antibody levels to 11 key P. vivax proteins in a village of western Thailand. Plasma samples from 546 volunteers enrolled in a cross-sectional survey conducted in 2012 in Kanchanaburi Province were utilized. Total IgG levels to 11 different proteins known or predicted to be involved in reticulocyte binding or invasion (ARP, GAMA, P41, P12, PVX_081550, and five members of the PvRBP family), as well as the leading pre-erythrocytic vaccine candidate (CSP) were measured using a multiplexed bead-based assay. Associations between IgG levels and infection status, age, and spatial location were explored. Individuals from a low-transmission region of western Thailand reacted to all 11 P. vivax recombinant proteins. Significantly greater IgG levels were observed in the presence of a current P. vivax infection, despite all infected individuals being asymptomatic. IgG levels were also higher in adults (18 years and older) than in children. For most of the proteins, higher IgG levels were observed in individuals living closer to the Myanmar border and further away from local health services. Robust IgG responses were observed to most proteins and IgG levels correlated with surrogates of exposure, suggesting these antigens may serve as potential biomarkers of exposure, immunity, or both. The online version of this article (doi:10.1186/s12936-017-1826-8) contains supplementary material, which is available to authorized users.