QUALITATIVE-ANALYSIS OF IMMUNE FUNCTION IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME - EVIDENCE FOR A SELECTIVE DEFECT IN SOLUBLE-ANTIGEN RECOGNITION
QUALITATIVE-ANALYSIS OF IMMUNE FUNCTION IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME - EVIDENCE FOR A SELECTIVE DEFECT IN SOLUBLE-ANTIGEN RECOGNITION
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DOI：
10.1056/nejm198507113130204
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发表时间：
1985-01-01
影响因子：
158.5
通讯作者：
FAUCI, AS
中科院分区：
文献类型：
--
作者：
LANE, HC;DEPPER, JM;FAUCI, AS
研究了获得性免疫缺陷综合征（AIDS）患者淋巴细胞的纯化亚群，以确定除淋巴细胞数量改变外，是否存在淋巴细胞功能的内在缺陷。促分裂原刺激的DNA合成，γ的产生干扰素、白细胞介素-2的产生和白细胞介素-2受体的表达，虽然在未分离的细胞群中减少，但在纯化的T细胞亚群中是正常的。DNA合成的可溶性蛋白抗原破伤风类毒素的反应，减少未分离和纯化的T细胞亚群。细胞混合实验表明，艾滋病患者的未分级淋巴细胞的低反应性不是由于主动抑制。艾滋病患者的淋巴细胞，虽然在有丝分裂刺激后能够进行正常程度的原始细胞转化和淋巴因子产生，但在识别和应答可溶性抗原的能力上存在内在缺陷。
Purified subpopulations of lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) were studied in order to determine whether intrinsic defects in lymphocyte function, aside from those due to alterations in lymphocyte numbers, were present. Mitogen-stimulated DNA synthesis, production of .gamma. interferon, production of interleukin-2, and expression of interleukin-2 receptors, although variably decreased in unseparated cell populations, were normal in populations of purified T-cell subsets. DNA synthesis in response to the soluble protein antigen tetanus toxoid was decreased in both unseparated and purified T-cell subpopulations. Cell-mixing experiments demonstrated that the hyporesponsiveness of the unfractionated lymphocytes from patients with AIDS was not due to active suppression. The lymphocytes of patients with AIDS, although capable of undergoing a normal degree of blast transformation and lymphokine production after mitogenic stimulation, have an intrinsic defect in their ability to recognize and respond to soluble antigen.