αvβ3 integrin expression in human odontoblasts and co-localization with osteoadherin
αvβ3 integrin expression in human odontoblasts and co-localization with osteoadherin
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DOI：
10.1177/154405910408300708
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发表时间：
2004-07-01
影响因子：
7.6
通讯作者：
Farges, JC
中科院分区：
文献类型：
--
作者：
Lucchini, M;Couble, ML;Farges, JC
整合素是异二聚体跨膜受体，其促进细胞粘附，从而有助于在正常和病理条件下维持组织组织结构。为了表征成牙本质细胞与其他细胞和人牙齿细胞外基质相互作用的方式，我们研究了骨粘附素的假定受体α v β 3整合素的表达。我们发现，α v β 3整合素的表达仅限于成牙本质细胞，血管，小圆形细胞在健全和龋牙髓。成牙本质细胞染色强度从根尖到牙尖区域逐渐增加。在整个成牙本质细胞层（在牙尖区略有减少）和前牙本质中，骨粘附素染色较强。体外分化的成牙本质细胞用抗α v β 3整联蛋白抗体染色，首先在细胞间接触的水平，然后在整个细胞膜。这些结果表明，α v β 3整合素可以发挥作用，在成牙本质细胞间的粘附和成牙本质细胞结合周围的前牙本质/牙本质/牙髓基质，可能通过骨粘附素。
Integrins are heterodimeric transmembrane receptors which promote cell adhesion, thus contributing to the maintenance of tissue organization in both normal and pathological conditions. To characterize the way odontoblasts may interact with other cells and the extracellular matrix in human teeth, we studied expression of alphavbeta3 integrin, a putative receptor for osteoadherin. We showed that alphavbeta3 integrin expression was restricted to odontoblasts, blood vessels, and small rounded cells in sound and carious pulp. Odontoblast staining intensity increased from the apical to the cusp region. Osteoadherin staining was strong in the whole odontoblast layer (with a slight decrease in the cusp region) and in predentin. Odontoblasts differentiating in vitro were stained with the anti-alphavbeta3 integrin antibody, first at the level of intercellular contacts, then throughout the cell membrane. These results suggest that the alphavbeta3 integrin could play a role in interodontoblast adhesion and odontoblast binding to the surrounding predentin/dentin/pulp matrix, possibly through osteoadherin.