Metabolic effects of dental resin components in vitro detected by NMR spectroscopy
Metabolic effects of dental resin components in vitro detected by NMR spectroscopy
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DOI：
10.1177/00220345010800030501
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发表时间：
2001-03-01
影响因子：
7.6
通讯作者：
Geurtsen, W
中科院分区：
文献类型：
--
作者：
Englemann, J;Leyhausen, G;Geurtsen, W
早期研究表明，共聚单体三乙二醇二甲基丙烯酸酯 (TEGDMA) 和光稳定剂 2-羟基-4-甲氧基二苯甲酮 (HMBP) 具有细胞毒性并抑制细胞生长。本研究的目的是通过核磁共振 (NMR) 光谱阐明 TEGDMA 和 HMBP 对永生接触抑制的瑞士白化小鼠胚胎细胞（3T3 成纤维细胞）的潜在代谢影响。使用 ED20 浓度的 TEGDMA 和 HMBP 孵育 24 小时后，通过 NMR 光谱分析细胞提取物和培养基的代谢变化。 TEGDMA 可在 3T3 细胞的所有组分（细胞质、脂质组分和培养基）中检测到，而 HMBP 仅在脂质组分中发现，与 TEGDMA（27 nmol/mg DNA）相比，以最大速率（51 nmol/mg DNA）积累。 TEGDMA 将磷酸单酯的浓度增加至对照的 180 +/- 36%，并将磷酸二酯的浓度降低至 65 +/- 5%（对照 = 100%）。因此，磷脂的周转增强，而膜的磷脂含量和组成没有显着改变。此外，TEGDMA改变了细胞的代谢状态，表现为三磷酸核苷的轻微降低和二磷酸核苷与三磷酸核苷的比率增加，而HMBP则没有影响。 TEGDMA 最显着的效果是细胞内谷胱甘肽水平几乎完全下降。对我们数据的分析表明，细胞-材料相互作用的核磁共振波谱可以揭示有机测试物质的代谢效应，而这是标准体外测定无法检测到的。共聚单体 TEGDMA 在不同水平上影响细胞的代谢，而 HMBP 在脂质部分中积累，对细胞代谢的影响明显较小。
Earlier studies have shown that the comonomer triethyleneglycol-dimethacrylate (TEGDMA) and the photostabilizer 2-hydroxy-4-methoxybenzophenone (HMBP) are cytotoxic and inhibit cell growth. It was the aim of this study to elucidate the underlying metabolic effects of TEGDMA and HMBP on immortal contact-inhibited Swiss albino mouse embryo cells (3T3 fibroblasts) by nuclear magnetic resonance (NMR) spectroscopy. Cell extracts and culture media were analyzed by NMR spectroscopy for metabolic changes after incubation for 24 hours with ED20-concentrations of TEGDMA and HMBP. TEGDMA could be detected in all fractions (cytosol, lipid fractions, and culture media) of 3T3 cells, while HMBP was found only in the lipid fraction accumulated at a maximum rate (51 nmol/mg DNA) compared with TEGDMA (27 nmol/mg DNA). TEGDMA increased the concentration of phosphomonoesters to 180 +/- 36% and decreased the phosphodiesters to 65 +/- 5% of controls (control = 100%). Thus, the turnover of phospholipids was enhanced, whereas content and composition of phospholipids of membranes did not alter markedly. Additionally, TEGDMA changed the metabolic state of cells, indicated by slight decreases of nucleoside triphosphates and an increase in the ratio of nucleoside diphosphates to nucleoside triphosphates, while HMBP had no effect. The most remarkable effect of TEGDMA was a nearly complete decline of the intracellular glutathione levels. Analysis of our data shows that NMR spectroscopy of cell-material interactions may reveal metabolic effects of organic test substances which are not detectable by standard in vitro assays. The comonomer TEGDMA affected the metabolism of the cells on different levels, while HMBP accumulated in the lipid fraction and induced significantly fewer effects on cell metabolism.