Cholesterol increases the L-type voltage-sensitive calcium channel current in arterial smooth muscle cells.
Cholesterol increases the L-type voltage-sensitive calcium channel current in arterial smooth muscle cells.
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胆固醇增加动脉平滑肌细胞中 L 型电压敏感钙通道电流。
DOI：
10.1161/01.res.71.4.1008
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发表时间：
1992
影响因子：
20.1
通讯作者：
Colucci,WS
中科院分区：
文献类型：
--
作者：
Sen,L;Bialecki,RA;Smith,E;Smith,TW;Colucci,WS
为了确定膜游离胆固醇是否影响血管平滑肌细胞中的钙电流，在通过暴露于富含胆固醇的脂质体使细胞胆固醇富集之前和之后进行全细胞膜片钳记录。暴露于富含胆固醇的脂质体导致L型电流在20小时内逐渐增加，并在20和32小时之间达到平台期（比基础增加73 +/- 7%）。该效应与灭活电位的突变和对二氢吡啶拮抗剂（-）-PN-202-791的敏感性降低相关。（+）-PN-202-791（一种二氢吡啶激动剂）刺激的最大L型电流无变化。脂质体暴露引起T型电流的短暂小幅增加（峰值效应，20分钟）。我们的结论是，膜胆固醇对血管平滑肌细胞中的L型钙电流具有重要影响，这很可能是由于通道功能状态的改变而不是通道表达的增加。
To determine whether membrane free cholesterol affects calcium currents in vascular smooth muscle cells, whole-cell patch clamp recordings were made before and after cholesterol enrichment of cells by exposure to cholesterol-rich liposomes. Exposure to cholesterol-rich liposomes resulted in a gradual increase in the L-type current over 20 hours and a plateau (73 +/- 7% increase over basal) between 20 and 32 hours. This effect was associated with a rightward shift in the inactivation potential and a decrease in the sensitivity to (-)-PN-202-791, a dihydropyridine antagonist. There was no change in the maximum L-type current stimulated by (+)-PN-202-791, a dihydropyridine agonist. Liposome exposure caused a small, transient increase in the T-type current (peak effect, 20 minutes). We conclude that membrane cholesterol has important effects on the L-type calcium current in vascular smooth muscle cells, which is most likely due to an alteration in channel functional state rather than an increase in channel expression.