An enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.
An enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.
复制标题
用于检测鸡体内禽腺病毒和禽腺病毒相关病毒抗体的酶联免疫吸附测定。
DOI：
10.2307/1589706
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发表时间：
1980
期刊：
影响因子：
1.4
通讯作者：
A. Pronovost
中科院分区：
文献类型：
--
作者：
G. Dawson;L. Orsi;V. J. Yates;P. Chang;A. Pronovost
采用酶联免疫吸附试验（ELISA）检测禽腺病毒（AV）和禽腺病毒相关病毒（A-AV）抗体。在暴露之前和之后，通过ELISA、病毒中和（VN）和免疫扩散（ID）测定经历自然和实验感染的鸡的血清中的CELO病毒和A-AV的抗体。发现ELISA系统在测定CELO病毒和A-AV的抗体浓度方面与VN相当。在许多情况下，发现ELISA比ID更敏感。
An enzyme-linked immunosorbent assay system (ELISA) was adapted for the detection of antibodies to avian adenovirus (AV) and avian adenovirus-associated virus (A-AV). Both before and after exposure, sera from chickens undergoing natural and experimental infections were assayed by ELISA, virus neutralization (VN), and immunodiffusion (ID) for antibody to both CELO virus and A-AV. The ELISA system was found to be comparable to VN for determining antibody concentrations to CELO virus and A-AV. In many cases, ELISA was found to be more sensitive than ID.