Development of a magnetic particle immunoassay for polybrominated diphenyl ethers and application to environmental and food matrices
Development of a magnetic particle immunoassay for polybrominated diphenyl ethers and application to environmental and food matrices
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DOI：
10.1016/j.chemosphere.2007.01.088
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发表时间：
2008-08-01
期刊：
影响因子：
8.8
通讯作者：
Rubio, Fernando M.
中科院分区：
文献类型：
--
作者：
Shelver, Weilin L.;Parrotta, Carmen D.;Rubio, Fernando M.
建立了一种灵敏的磁微粒酶联免疫分析法（ELISA），用于水、牛奶、鱼和土壤样品中多溴联苯醚（PBDEs）的分析。该方法快速，可用于分析样品净化后约1小时内的50个样品。该检测方法对以下溴化二苯醚同系物的检测限低于0.1 ppb：BDE-47、BDE-99、BDE-28、BDE-100和BDE-153，检测限与GC-NCI-MS大致相同。BDE-153相对于BDE-47的含量低于15%。正如预期的那样，灵敏度与半抗原结构和BIDE同源物结构之间的相似性成比例。一些与半抗原结构相似的含氧同源物显示出高至中度的交叉反应性。其他多溴二苯醚或氯化环境污染物的交叉反应性非常低。该测定法从加标水样中回收多溴联苯醚的效果良好，日内和日间差异很小。与GC-NCI-MS法比较，ELISA法的精密度和灵敏度相当，回收率更高。GC-NCI-MS方法的回收率较低可能是由于使用了内标物而不是同位素取代的材料（由于该方法观察到的裂解模式而无法使用）。在ELISA之前的净化方法是基质依赖性的，不需要对环境水样进行预处理，而鱼、牛奶和土壤样品需要不同程度的净化。通过ELISA和GC-MS对各种环境样品进行分析表明，ELISA为筛选各种样品中的多溴联苯醚提供了一种及时和具有成本效益的方法。(C)2008爱思唯尔有限公司保留所有权利。
A sensitive magnetic particle enzyme-linked immunoassay (ELISA) was developed to analyze polybrominated diphenyl ethers (PBDEs) in water, milk, fish, and soil samples. The assay was rapid and can be used to analyze fifty samples in about 1 h after sample cleanup. The assay has a limit of detection (LOD) below 0.1 ppb towards the following brominated diphenyl ether (BDE) congeners: BDE-47, BDE-99, BDE-28, BDE-100, and BDE-153, with the LOD approximately the same as GC-NCI-MS. The congeners most readily recognized in the ELISA were BDE-47 and BDE-99 with the cross-reactivitys of BDE-28, BDE-100, and BDE-153 being less than 15% relative to BDE-47. As anticipated, the sensitivities are proportional to the similarities between the hapten structure and the BIDE congener structure. Some oxygenated congeners with structural similarity to the hapten showed high to moderate cross-reactivities. Very low cross-reactivity was observed for other PBDEs or chlorinated environmental contaminants. The assay gave good recoveries of PBDEs from spiked water samples and a very small within and between day variance. Comparison with GC-NCI-MS demonstrated the ELISA method showed equivalent precision and sensitivity, with better recovery. The lower recovery of the GC-NCI-MS method could be caused by the use of an internal standard other than an isotopically substituted material that could not be used because of the fragmentation pattern observed by this method. The cleanup methods prior to ELISA were matrix dependent, no pretreatment was needed for environmental water samples, while fish, milk, and soil samples required various degrees of cleanup. Analysis of this wide variety of environmental samples by both ELISA and GC-MS demonstrated ELISA provides a timely and cost-effective method to screen for PBDEs in a variety of samples. (C) 2008 Elsevier Ltd. All rights reserved.