Phospholipase C and adenylate cyclase signalling systems in the action of hCG on porcine myometrial smooth muscle cells.
Phospholipase C and adenylate cyclase signalling systems in the action of hCG on porcine myometrial smooth muscle cells.
复制标题
hCG 对猪子宫肌层平滑肌细胞作用中的磷脂酶 C 和腺苷酸环化酶信号系统。
DOI：
10.1677/joe.0.1480175
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
A. Ziecik
中科院分区：
文献类型：
--
作者：
J. Kisielewska;A. Flint;A. Ziecik
虽然子宫是LH及其同系物hCG的靶组织，但子宫肌层细胞中对LH/hCG应答的第二信使系统尚未建立。在这项研究中，我们研究了蛋白激酶A和蛋白激酶C在hCG对猪子宫平滑肌细胞的作用中的作用。子宫肌层取自卵巢切除的小母猪，连续5天给予2.5 mg苯甲酸雌二醇加50 mg孕酮。培养子宫肌层细胞48 h后加入不同剂量的hCG。在持续24 h的孵育中，增加剂量的hCG刺激[3 H]肌醇磷酸（IP）积累的浓度依赖性增加。最高剂量的hCG（1000 mU/ml）使IP的周转率增加2.4倍，反映在IP 1、IP 2和IP 3的升高中，并且用去甲肾上腺素观察到类似的作用。hCG对IP积累的时间和浓度依赖性效应发生在孵育16和24 h之间。用最低剂量的hCG（0.1和1 mU/ml）孵育心肌细胞引起cAMP积累的显著增加，但最高剂量（10-1000 mU/ml）对cAMP浓度没有影响。这是第一次证明LH/hCG受体信号传导导致子宫肌层细胞中磷酸肌醇周转增加以及cAMP生成，并得出结论，蛋白激酶A和蛋白激酶C信号传导机制均参与促性腺激素在猪子宫肌层平滑肌细胞中的作用。
Although the uterus is a target tissue for LH and its homologue hCG the second messenger system responding to LH/hCG in myometrial cells is not established. In this study we investigated the involvement of protein kinase A and protein kinase C in the action of hCG on porcine myometrial smooth muscle cells in vitro. Myometrium was obtained from ovariectomized gilts given 2.5 mg oestradiol benzoate plus 50 mg progesterone for five consecutive days. Myometrial cells were cultured for 48 h and different doses of hCG were then added. Increasing doses of hCG stimulated concentration-dependent increases in [3H]inositol phosphates (IPs) accumulation in incubations lasting 24 h. The highest dose of hCG (1000 mU/ml) increased turnover of IPs by 2.4-fold as reflected in elevations in IP1, IP2 and IP3, and similar effects were observed with noradrenaline. The time- and concentration-dependent effects of hCG on IPs accumulation occurred between 16 and 24 h of incubation. Incubation of myocytes with the lowest doses of hCG (0.1 and 1 mU/ml) caused a significant increase in cAMP accumulation but the highest doses (10-1000 mU/ml) had no effect on cAMP concentrations. This is the first demonstration that LH/hCG receptor signalling leads to increased inositol phosphate turnover in myometrial cells as well as cAMP generation and it leads to the conclusion that both protein kinase A and protein kinase C signalling mechanisms are involved in gonadotrophin action in porcine myometrial smooth muscle cells.