Three aromatic amino acids in gastric juice as potential biomarkers for gastric malignancies
Three aromatic amino acids in gastric juice as potential biomarkers for gastric malignancies
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胃液中的三种芳香氨基酸作为胃恶性肿瘤的潜在生物标志物
DOI：
10.1016/j.aca.2011.03.053
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发表时间：
2011-05-23
影响因子：
6.2
通讯作者：
Na, Renhua
中科院分区：
文献类型：
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作者：
Deng, Kai;Lin, Sanren;Na, Renhua
对于早期胃恶性肿瘤的筛查，现有血清生物标志物的敏感性和特异性有限。胃液生物标志物缺乏，需要进一步研究。我们将寻找潜在生物标志物的研究分为四个部分：(1)利用荧光光谱和HPLC检测患者胃液中的差异荧光光谱和峰，(2)利用LC/MS和NMR鉴定和验证差异峰，(3)潜在生物标志物的定量，(4)建立诊断检测方法。胃恶性肿瘤患者胃液中荧光强度（FI）、酪氨酸、苯丙氨酸、色氨酸和总蛋白含量均显著升高（P < 0.01）。P均< 0.001，受试者工作特征曲线下面积为酪氨酸，0.838；苯丙氨酸,0.856;色氨酸，0.816。苯丙氨酸检测胃恶性肿瘤的特异性为79.4%，敏感性仅为87.9%。胃液中芳香族氨基酸可作为潜在的诊断性生物标志物筛选胃恶性肿瘤。与胃活检相比，这是一种微创且经济的方法。(C) 2011 Elsevier B.V.版权所有
For screening early-stage gastric malignancies, the existing serum biomarkers have limited sensitivity and specificity. Gastric juice biomarkers are scarce and require further investigation. We divided this study on searching potential biomarkers into four parts: (1) detection of differential fluorescence spectrum and peaks in the gastric juice from patients using fluorescence spectroscopy and HPLC, (2) identification and validation of differential peaks using LC/MS and NMR, (3) quantification of potential biomarkers, and (4) establishment of diagnostic detection. The fluorescence intensity (FI), tyrosine, phenylalanine, tryptophan and total protein content were significantly higher in the gastric juice of patients with gastric malignancies (all P < 0.01). With all P < 0.001, the areas under the receiver operating characteristic curves of the biomarkers were tyrosine, 0.838; phenylalanine, 0.856; and tryptophan, 0.816. At a specificity of 79.4%, the sensitivity for gastric malignancy detection with phenylalanine was 87.9% only. Aromatic amino acids in gastric juices could be used as potential diagnostic biomarkers to screen gastric malignancies. It is a less-invasive and economical method compared to gastric biopsy. (C) 2011 Elsevier B.V. All rights reserved.