A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum x Gossypium barbadense backcross population
A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum x Gossypium barbadense backcross population
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作者：
Lacape, JM;Nguyen, TB;Hau, B
利用1014个分子标记对陆地棉×海岛棉回交群体的75个BC 1单株进行了分析。该图谱由888个位点组成，包括465个AFLP位点、229个SSR位点、192个RFLP位点和2个形态学标记，分布在37个连锁群中，代表了26条染色体的大部分，总长度为4400 cM。本文提出了2n = 4x = 52四倍体棉花基因组中13对同源异交A/D染色体的部分修正表。主要的修订涉及c3-c17，c4-c22，c5-D 08和c10-c20同源对，是基于已知染色体分配的68个SSR和RFLP位点的定位，以及与先前发表的G. hirsutum x G.巴巴多斯地图。在这些地图中的基因座的顺序和常见的SSR和RFLP基因座的距离的整体一致性，允许估计的共识长度，达到最低5500厘米，是令人鼓舞的，为未来的努力，旨在开发一个综合地图的栽培棉。本图谱也为棉花数量性状孟德尔成分的精确定位提供了坚实的框架。
An interspecific Gossypium hirsutum x Gossypium barbadense backcross population of 75 BC1 plants was evaluated for 1014 markers. The map consists of 888 loci, including 465 AFLPs, 229 SSRs, 192 RFLPs, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altogether spanning 4400 cM Loci were not evenly distributed over linkage groups, and 18 of the 26 long groups had a single dense region. This paper proposes a partially revised list of the 13 pairs of homoeologous A/D chromosomes of the 2n = 4x = 52 tetraploid cotton genome. The major revisions, which involve the c3-c17, c4-c22, c5-D08, and c10-c20 homocologous pairs, are based on the mapping of 68 SSR and RFLP loci with a known chromosome assignment, as well as on comparative alignments with previously published G. hirsutum x G. barbadense maps. The overall congruency in the locus orders and distances of common SSR and RFLP loci in these maps allows for an estimation of the consensus length that reaches a minimum of 5500 cM, and is encouraging for future efforts aimed at developing an integrated map of cultivated cotton. The present map also provides a firm framework for precision mapping of Mendelian components of quantitative traits in cotton.