Manganese oxide nanoparticles induce genotoxicity and DNA hypomethylation in the moss Physcomitrella patens
Manganese oxide nanoparticles induce genotoxicity and DNA hypomethylation in the moss Physcomitrella patens
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DOI：
10.1016/j.mrgentox.2018.12.006
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发表时间：
2019-06-01
影响因子：
1.9
通讯作者：
Mukherjee, Anita
中科院分区：
文献类型：
--
作者：
Ghosh, Ilika;Sadhu, Abhishek;Mukherjee, Anita
纳米颗粒的遗传毒性是一个主要的问题，纳米安全性评估的brabrites，因为他们是主要的殖民者裸露的土地，大气污染的指标和良好的痕量金属。本研究首次证实MnONP对小立碗藓（Physcomitrelapatens）的植物体内遗传毒性，小立碗藓是一种用于进化发育遗传学的模式植物。彗星试验证实在所有测试浓度下诱导DNA链断裂，证实了ROS的产生增加，Mn溶解，摄取和内化增加。遗传毒性通常与表观遗传改变相结合。在本研究中，使用同分异构限制性内切酶HpaII（消化未甲基化和半甲基化的DNA）和MspI（消化5 '-CmCGG-3 '处的甲基化DNA），通过甲基化敏感彗星试验研究了单细胞水平的整体DNA甲基化模式。MnONP在用最高浓度的MnONP（20 μ g/mL）处理的展叶商陆配子体中引发DNA低甲基化。MnONP暴露后引起的DNA低甲基化与DNA甲基化阻断剂5-氮杂胞苷相当。这可能是由于纳米材料通过形成H2 O2、OH中心点和O-2（中心点-）介导的致染色体断裂作用。目前还没有利用DNA损伤和DNA甲基化检测纳米材料在植物中的表观遗传毒性的报道。这可以开辟新的研究途径，评估环境相关的纳米颗粒的表观遗传毒性的影响，使用branchites作为模式指示植物系统。
The genotoxicity of nanoparticles is a major concern for nano-safety appraisal in the bryophytes as they are the primary colonizers of bare land, indicators of atmospheric pollution and excellent accumulators of trace metals. The present study for the first time evince the in planta genotoxicity of MnONP in Physcomitrella patens a model plant system utilized for evolutionary developmental genetics. The induction of DNA strand breaks was confirmed by comet assay at all tested concentrations corroborated with the enhanced generation of ROS, increase in Mn dissolution, uptake and internalization.Genotoxicity is often coupled with epigenetic alterations. In the present study, global DNA methylation pattern at the level of single cells was studied by the methylation sensitive comet assay using the isochizomeric restriction endonucleases HpaII (digests unmethylated and hemimethylated DNA) and MspI (digests methylated DNA at 5 '-CmCGG-3 '). MnONP incited DNA hypomethylation in P. patens gametophores treated with the highest concentration of MnONP (20 mu g/mL). The DNA hypomethylation incurred upon MnONP exposure was comparable with that of the DNA methylation blocker 5-azacytidine. This can be ascribed to its clastogenic potential mediated by the formation of H2O2, OH center dot and O-2(center dot-).There are no reports on the epigenotoxicity of nanomaterials in plants utilizing the detection of DNA damage and DNA methylation. This can open up new avenues of research on the assessment of the epigenotoxic impact of environmentally relevant nanoparticles using bryophytes as model indicator plant system.