REGIONAL MESSENGER-RNA CHANGES IN BRAIN-STEM MOTOR NEURONS FROM PATIENTS WITH AMYOTROPHIC-LATERAL-SCLEROSIS
REGIONAL MESSENGER-RNA CHANGES IN BRAIN-STEM MOTOR NEURONS FROM PATIENTS WITH AMYOTROPHIC-LATERAL-SCLEROSIS
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DOI：
10.1007/bf03160014
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发表时间：
1992-12-01
期刊：
影响因子：
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通讯作者：
SUN, DY
中科院分区：
文献类型：
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作者：
HARTMANN, HA;SUN, DY
用S-35在3'端标记来自mRNA的中等大小神经丝蛋白（NFM）的反义寡核苷酸（54 mer），并通过聚丙烯酰胺凝胶电泳（PAGE）纯化。对肌萎缩侧索硬化（ALS）患者延髓切片进行原位杂交。将玻片浸入感光乳剂中，显影并染色，舌下神经核和疑核的神经元显示与正常神经元相比银颗粒明显减少。这表明mRNA的减少，这可能先于核糖体RNA的减少和神经丝蛋白的变化，这些变化已经被ALS中的一些研究者描述。它并没有解决mRNA的减少是由于转录减少还是mRNA衰变增加的问题。
An antisense oligonucleotide (54 mer) from the mRNA to the mid-size neurofilament protein (NFM) was labeled with S-35 on the 3' end and purified,by polyacrylamide gel electrophoresis (PAGE). In situ hybridization was performed on sections from medulla oblongata of patients with amyotrophic lateral sclerosis (ALS). The slides were dipped in photographic emulsion, developed, and stained.Neurons from both nucleus hypoglossus and nucleus ambiguus showed a marked reduction of silver grains when compared to normal. This indicates a reduction of mRNA, which may precede the reduction of ribosomal RNA and the changes in neurofilament proteins that have been described by several investigators in ALS. It does not settle the question of whether the reduction of mRNA is owing to reduced transcription or increased decay of mRNA.