Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
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DOI：
10.3390/nu11112802
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发表时间：
2019-11-01
期刊：
影响因子：
5.9
通讯作者：
Shibata, Shigenobu
中科院分区：
文献类型：
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作者：
Sasaki, Hiroyuki;Miyakawa, Hiroki;Shibata, Shigenobu
众所周知，水溶性膳食纤维可以调节粪便微生物区系。虽然有一些研究纤维摄入时机对代谢影响的报道，但还没有关于摄入时机对微生物区系影响的报道。因此，在这项研究中，我们研究了菊粉食物对粪便微生物区系的时间效应。小鼠被安置在每天两餐的条件下，早上禁食时间较长，晚上禁食时间较短。在摄入菊粉后10~14d，收集盲肠内容物和粪便，测定盲肠pH值和短链脂肪酸(SCFAs)。用16S rDNA测序法测定微生物组。早晨饲喂菊粉而不是晚上饲喂菊粉降低了盲肠的pH值，增加了SCFAs，并改变了微生物群的组成。这些数据表明，菊糖在活动期比非活动期更容易被粪便微生物区系消化。此外，为了证实禁食时间的影响，小鼠被安置在一天一餐的时间表下。当禁食时间相等时，早晚之间的差异几乎消失。因此，我们的研究表明，早餐摄入菊粉，通常是在较长的禁食时间之后，对微生物区系有更大的影响。
Water-soluble dietary fiber is known to modulate fecal microbiota. Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota. Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota. Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening. Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured. The microbiome was determined using 16S rDNA sequencing. Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition. These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period. Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule. When the duration of fasting was equal, the difference between morning and evening nearly disappeared. Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.