Enhanced apoptosis and transforming growth factor-β1 expression in colorectal adenomas and carcinomas after sulindac therapy
Enhanced apoptosis and transforming growth factor-β1 expression in colorectal adenomas and carcinomas after sulindac therapy
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舒林酸治疗后结直肠腺瘤和癌中细胞凋亡和转化生长因子-β1 表达增强
DOI：
10.1007/bf02235490
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发表时间：
2001
影响因子：
3.9
通讯作者：
N. Nagasue
中科院分区：
文献类型：
--
作者：
R. Masunaga;H. Kohno;D. Dhar;T. Kotoh;H. Tabara;M. Tachibana;H. Kubota;N. Nagasue
目的：探讨舒林酸对人大肠癌的治疗作用。方法：对33例散发性结直肠癌患者（舒林达克组）术前2周给予舒林达克（300 mg/d）治疗。切除标本采用末端dUTP缺口末端标记法检测细胞凋亡，免疫组化检测转化生长因子(TGF)-β1表达。结果与历史对照组的结果进行比较。我们还检测了29例舒林达克处方前的癌术前活检，以及22例切除标本中并发结直肠腺瘤（舒林达克组9例，对照组13例）TGF-β1的表达。结果：在切除的癌和腺瘤中，舒林达克组细胞凋亡发生率高于对照组，TGF-β1评分高于对照组。TGF-β1过表达与细胞凋亡发生在腺瘤组织的同一区域，而在癌组织中不存在。TGF-β1评分与细胞凋亡频率在腺瘤中呈显著正相关（P=0.01,ρ=0.91），在癌中无显著正相关（P=0.89,ρ=0.03）。结论：舒林酸可诱导人结直肠癌及腺瘤细胞凋亡。此外，舒林酸的抗肿瘤作用之一可能是通过上调TGF-β1的表达介导的，特别是在结直肠腺瘤中。
PURPOSE: We tried to elucidate the effects of sulindac on human colorectal carcinoma. METHODS: Sulindac (300 mg/day) was administered for two weeks before operation to 33 patients with sporadic colorectal carcinoma (Sulindac Group). Resected specimens were used to detect apoptosis by terminal dUTP nick end labeling and transforming growth factor (TGF)-β1 expression by immunohistochemistry. The results were compared with those from the historical Control Group. Twenty-nine available preoperative biopsies taken from carcinomas before sulindac prescription and 22 concurrent colorectal adenomas (9 and 13 in Sulindac and Control Groups, respectively) in the resected specimen were also examined regarding TGF-β1 expression. RESULTS: In the resected carcinomas and adenomas, more frequent apoptosis and higher TGF-β1 scores were observed in the Sulindac Group than in the Control Group. Overexpression of TGF-β1 and apoptosis occurred in the same region in adenomas but not in carcinomas. A positive correlation between TGF-β1 scores and apoptotic frequency was found in adenomas (P=0.01,ρ=0.91) but not in carcinomas (P=0.89,ρ=0.03). CONCLUSION: We conclude that sulindac induces apoptosis in human colorectal carcinomas as well as in adenomas. Also, one of the antineoplastic effects of sulindac might be mediated by upregulating TGF-β1 expression, particularly in colorectal adenomas.
自闭症患者血清转化生长因子-槟榔水平降低
DOI：
--
发表时间：
2007
期刊：
Prog Neuropsychopharmacol Biol Psychiatry 31(1)
影响因子：
--
作者：
Okada;K.;et. al.
通讯作者：
et. al.