Trim5α protein restricts both HIV-1 and murine leukemia virus
Trim5α protein restricts both HIV-1 and murine leukemia virus
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DOI：
10.1073/pnas.0402876101
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发表时间：
2004-07-20
影响因子：
11.1
通讯作者：
Stoye, JP
中科院分区：
文献类型：
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作者：
Yap, MW;Nisole, S;Stoye, JP
HIV-1和嗜n型小鼠白血病病毒（N-MLV）的复制在许多不同的灵长类细胞中受到限制。在一些细胞系中，交叉饱和实验表明这两种病毒与相同的限制性因子相互作用。最近，人们发现恒河猴的trim5 α蛋白可以抑制HIV-1。我们已经证实了这一结果，并表明来自两个非洲绿猴细胞系Vero和CV-1的Trim5alpha也限制了HIV-1。此外，我们发现人类、恒河猴和非洲绿猴的Trim5alpha可以抑制N-MLV。通过使用一组MLV衣壳突变体，在不同的灵长类动物Trim5alpha cdna中鉴定出抗MLV活性的细微差异。从人类和绿猴中分离的Trim1也被发现限制N-MLV。我们假设Trim蛋白家族在对病毒感染的先天免疫中起着广泛的作用。
Replication of HIV-1 and N-tropic murine leukemia virus (N-MLV) is restricted in a number of different primate cells. In some cell lines, cross-saturation experiments suggest that the two viruses are interacting with the same restriction factor. Recently, Trim5alpha protein from rhesus monkey was found to restrict HIV-1. We have confirmed this result and have shown that Trim5alpha from two African green monkey cell lines, Vero and CV-1, also restricts HIV-1. In addition, we show that human, rhesus, and African green monkey Trim5alpha can restrict N-MLV. By using a panel of MLV capsid mutants, subtle differences in the anti-MLV activity were identified among the different primate Trim5alpha cDNAs. Trim1 isolated from humans and green monkeys was also found to restrict N-MLV. We hypothesize that the Trim family of proteins plays a widespread role in innate immunity to viral infection.