Comparison of the fecal microbiota of dholes high-throughput Illumina sequencing of the V3-V4 region of the 16S rRNA gene
Comparison of the fecal microbiota of dholes high-throughput Illumina sequencing of the V3-V4 region of the 16S rRNA gene
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16S rRNA 基因 V3-V4 区域高通量 Illumina 测序比较豺粪便微生物群
DOI：
10.1007/s00253-015-7257-y
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发表时间：
2016-04-01
影响因子：
5
通讯作者：
Tu, Xiangyu
中科院分区：
文献类型：
--
作者：
Wu, Xiaoyang;Zhang, Honghai;Tu, Xiangyu
肠道微生物是复杂生态系统的一部分。它们与宿主有着相互的关系，对维持宿主的健康起着至关重要的作用。为了优化饲养策略并改善濒危物种豺的健康状况，我们使用针对 16S rRNA 基因 V3-V4 区域的高通量 Illumina 测序分析了 4 只圈养豺的粪便微生物结构。多样性指数和稀疏曲线表明四只野狗肠道内微生物多样性较高。四个样本中操作分类单元（OTU）的平均数量为1196个，但所有文库共有的OTU数量为126个，表明只有少数优势种。系统发育分析从16S rRNA基因序列中鉴定出19个原核生物门，其中只有5个门是核心微生物群：拟杆菌门（21.63-38.97%）、厚壁菌门（20.97-44.01%）、变形菌门（9.33-17.60%）、梭杆菌门（9.11-17.90） %) 和放线菌 (1.22-2.87 %)。这五个门占除一门之外所有豺中细菌的97%，其中78%的细菌来自这五个门。我们的研究结果为了解与保护这一濒危物种相关的生物学机制提供了有效的理论基础。
Intestinal microbes are part of a complex ecosystem. They have a mutual relationship with the host and play an essential role in maintaining the host's health. To optimize the feeding strategies and improve the health status of the dhole, which is an endangered species, we analyzed the structure of fecal microbes in four captive dholes using high-throughput Illumina sequencing targeting the V3-V4 region of the 16S rRNA gene. The diversity indexes and rarefaction curves indicated high microbial diversity in the intestines of the four dholes. The average number of operational taxonomical units (OTUs) in the four samples was 1196, but the number of OTUs common to all libraries was 126, suggesting only a few dominant species. Phylogenetic analysis identified 19 prokaryotic phyla from the 16S rRNA gene sequences, of which only 5 phyla were core microbiota: Bacteroidetes (21.63-38.97 %), Firmicutes (20.97-44.01 %), Proteobacteria (9.33-17.60 %), Fusobacteria (9.11-17.90 %), and Actinobacteria (1.22-2.87 %). These five phyla accounted for 97 % of the bacteria in all the dholes apart from one, in which 78% of the bacteria were from these phyla. The results of our study provide an effective theoretical basis from which to reach an understanding of the biological mechanisms relevant to the protection of this endangered species.