Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
复制标题
恒河猴树突状细胞可独立于 DC-SIGN 有效传播灵长类慢病毒。
DOI：
10.1073/pnas.032654399
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发表时间：
2002
影响因子：
11.1
通讯作者：
KewalRamani,VineetN
中科院分区：
文献类型：
--
作者：
Wu,Li;Bashirova,ArmanA;Martin,ThomasD;Villamide,Loreley;Mehlhop,Erin;Chertov,AndreiO;Unutmaz,Derya;Pope,Melissa;Carrington,Mary;KewalRamani,VineetN
在这里，我们描述了人DC-SIGN，树突状细胞特异性C型凝集素的恒河猴同源物的分离和表征。mac-DC-SIGN与hu-DC-SIGN有92%的相同性。mac-DC-SIGN保留了hu-DC-SIGN的病毒传播功能，捕获并有效转导猿猴和人类免疫缺陷病毒以靶向CD 4 +T细胞。然而，令人惊讶的是，mac-DC-SIGN在恒河猴树突状细胞捕获和传播灵长类慢病毒的能力中没有发挥可辨别的作用。表达和中和分析表明，这一过程是DC-SIGN独立的猕猴，虽然不能排除其他凝集素分子的参与。灵长类慢病毒在病毒传播中有效利用人类和恒河猴树突状细胞而不直接感染细胞的能力表明，这些病毒利用了保守的树突状细胞机制，其中DC-SIGN家族分子是重要的贡献者，但不是唯一的参与者。
Here, we describe the isolation and characterization of the rhesus macaque homolog for human DC-SIGN, a dendritic cell-specific C-type lectin. mac-DC-SIGN is 92% identical to hu-DC-SIGN. mac-DC-SIGN preserves the virus transmission function of hu-DC-SIGN, capturing and efficiently transducing simian and human immunodeficiency virus to target CD4+T cells. Surprisingly, however, mac-DC-SIGN plays no discernable role in the ability of rhesus macaque dendritic cells to capture and transmit primate lentiviruses. Expression and neutralization analyses suggest that this process is DC-SIGN independent in macaque, although the participation of other lectin molecules cannot be ruled out. The ability of primate lentiviruses to effectively use human and rhesus dendritic cells in virus transmission without the cells becoming directly infected suggests that these viruses have taken advantage of a conserved dendritic cell mechanism in which DC-SIGN family molecules are significant contributors but not the only participants.