Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin
Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin
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玻利维亚亚马逊河流域的多种蚊子特异性黄病毒
DOI：
10.1099/jgv.0.001518
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发表时间：
2021
影响因子：
3.8
通讯作者：
Sawa Hirofumi
中科院分区：
文献类型：
--
作者：
Orba Yasuko;Matsuno Keita;Nakao Ryo;Kryukov Kirill;Saito Yumi;Kawamori Fumihiko;Loza Vega Ariel;Watanabe Tokiko;Maemura Tadashi;Sasaki Michihito;Hall William W.;Hall Roy A.;Pereira Juan Antonio;Nakagawa So;Sawa Hirofumi
黄病毒属除了众所周知的医学上重要的虫媒病毒外，还包括一系列蚊子特有的病毒。对玻利维亚采集的蚊子中的病毒进行分离和全面的基因组分析，确定了三种新的黄病毒种类。补骨脂黄病毒(Psfv)是从白色补骨脂中分离到的一种病毒。其编码序列与伊蚊相关谱系II昆虫特异性黄病毒Marisma病毒的编码序列有60 %的同源性。分离的PSFV在白纹伊蚊和埃及伊蚊来源的细胞中复制，但在哺乳动物Vero或BHK-21细胞系中不复制。另外两种黄病毒--肩黄病毒(Ochlerotatus sharularis flaviVirus，OSFV)和黄病毒(Mansonia flaviVirus，MAFV)，分别从肩隆棘鱼和虎尾曼松黄病毒中分离得到，它们与经典的ISF谱系IISF同源。这些病毒的蛋白质编码序列与已知的亲缘关系最近的IISF，包括西双版纳伊蚊黄病毒和沙贝特黄病毒，分别只有60%和40% 的同源性。系统发育分析表明，MAFV与目前已知的库蚊亚科相关谱系I ISF的群体明显不同。有趣的是，MAFV衣壳蛋白的预测氨基酸序列大约是任何其他已知黄病毒的两倍。我们的结果表明，在玻利维亚亚马逊盆地边缘的地点可以发现具有鲜明特征的黄病毒，这些地点也是叮咬人的蚊子密集种群的家园。
The genusFlavivirusincludes a range of mosquito-specific viruses in addition to well-known medically important arboviruses. Isolation and comprehensive genomic analyses of viruses in mosquitoes collected in Bolivia resulted in the identification of three novel flavivirus species. Psorophora flavivirus (PSFV) was isolated fromPsorophora albigenu. The coding sequence of the PSFV polyprotein shares 60 % identity with that of theAedes-associated lineage II insect-specific flavivirus (ISF), Marisma virus. Isolated PSFV replicates in bothAedes albopictus- andAedes aegypti-derived cells, but not in mammalian Vero or BHK-21 cell lines. Two other flaviviruses, Ochlerotatus scapularis flavivirus (OSFV) and Mansonia flavivirus (MAFV), which were identified fromOchlerotatus scapularisandMansonia titillans,respectively, group with the classical lineage I ISFs. The protein coding sequences of these viruses share only 60 and 40 % identity with the most closely related of known lineage I ISFs, including Xishuangbanna aedes flavivirus and Sabethes flavivirus, respectively. Phylogenetic analysis suggests that MAFV is clearly distinct from the groups of the current knownCulicinae-associated lineage I ISFs. Interestingly, the predicted amino acid sequence of the MAFV capsid protein is approximately two times longer than that of any of the other known flaviviruses. Our results indicate that flaviviruses with distinct features can be found at the edge of the Bolivian Amazon basin at sites that are also home to dense populations of human-biting mosquitoes.