Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids
Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids
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DOI：
10.1186/s13287-018-0943-9
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发表时间：
2018-07-11
影响因子：
7.5
通讯作者：
Zambrano, Alberto
中科院分区：
文献类型：
--
作者：
Magro-Lopez, Esmeralda;Palmer, Charlotte;Zambrano, Alberto
来自人类多能干细胞的类器官正在成为器官发育、药物筛选、再生医学和疾病建模研究的合适模型。在基质胶培养的三维minilungs最近已产生的人胚胎干细胞。这些特殊的类器官，称为肺芽类器官，显示出分支气道和早期肺泡结构，类似于人类妊娠中期肺部的结构。我们在这里表明，用含有地塞米松的肺和气道上皮成熟混合物治疗此类类器官可以促使肺芽类器官形成球拍状结构。这种策略可能有助于增加肺类器官的多功能性，并产生比原始分支纹理更先进的结构。
Organoids from human pluripotent stem cells are becoming suitable models for studies of organ development, drug screening, regenerative medicine, and disease modeling. Three-dimensional minilungs in Matrigel culture have recently been generated from human embryonic stem cells. These particular organoids, named lung bud organoids, showed branching airway and early alveolar structures resembling those present in lungs from the second trimester of human gestation. We show here that the treatment of such organoids with a lung and airway epithelial maturation cocktail containing dexamethasone drives lung bud organoids to the formation of paddle-racquet like structures. This strategy may help to increase the versatility of lung organoids and to generate structures more advanced than the original branching texture.