Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells.
Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells.
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沙眼衣原体性病淋巴肉芽肿原体的纯化及其与 HeLa 细胞的相互作用。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Paul
中科院分区：
文献类型：
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作者：
S. Bose;R. Paul
建立了一种制备性淋巴肉芽肿沙眼衣原体LGV 434和404株感染性基本体的方法，该基本体在HeLa 229细胞内生长期间被标记。在聚碳酸酯薄膜过滤的样品通过蔗糖梯度进行速度沉降后获得的最终制备不含宿主蛋白，更重要的是，不含衣原体网状小体。使用这些纯化的制剂，发现LGV 434基本体与HeLa 229培养物的结合不受多种凝集素或产气荚膜梭菌和霍乱弧菌神经氨酸酶的影响。这种结合可被硫酸葡聚糖和温和的胰酶处理的HeLa细胞所抑制。用胰酶、胰凝乳酶、神经氨酸酶以及各种碳水化合物和凝集素处理纯化的基本体，不会对HeLa培养的结合率产生任何影响。热灭活的基本体与宿主细胞结合的能力明显降低。
A procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains LGV 434 and 404 of Chlamydia trachomatis, labelled during intracellular growth in HeLa 229 cells. The final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. Using such purified preparations, it was found that the association of LGV 434 elementary bodies with HeLa 229 cultures was unaffected by the pretreatment of the host cells with a variety of lectins or with neuraminidases from Clostridium perfringens and Vibrio cholerae. The association was inhibited by dextran sulphate and by mild trypsin treatment of HeLa cultures. Treatment of purified elementary bodies with trypsin, chymotrypsin, neuraminidases and a variety of carbohydrates and lectins did not produce any change in the rate of association with HeLa cultures. Heat-inactivated elementary bodies were significantly less able to associate with the host cells.