Simazine perturbs the maturational competency of mouse oocyte through inducing oxidative stress and DNA damage
Simazine perturbs the maturational competency of mouse oocyte through inducing oxidative stress and DNA damage
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DOI：
10.1016/j.ecoenv.2021.113105
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发表时间：
2021-12-23
影响因子：
6.8
通讯作者：
Duan, Xing
中科院分区：
文献类型：
--
作者：
Shang, Jian-Zhou;Li, Shi-Ru;Duan, Xing
西玛津是一种三嗪农药，通常在地下水和土壤中检测到，据报道可能影响人类和动物的生殖健康。然而，西玛津对女性生殖细胞发育的影响仍不清楚。在本研究中，我们观察到西玛津暴露降低卵母细胞成熟能力和胚胎发育能力。重要的是，西玛津暴露破坏微管稳定性和肌动蛋白聚合，导致纺锤体组装和迁移失败。此外，西玛津暴露损害线粒体功能和胞质Ca 2+稳态在卵母细胞和2-细胞胚胎，从而增加活性氧（ROS）的水平。此外，西玛津暴露诱导卵母细胞成熟过程中的DNA损伤和早期凋亡。总的来说，我们的结果表明西玛津诱导的线粒体功能障碍和凋亡是卵母细胞质量差的主要原因。
Simazine is a triazine pesticides that typically detected in ground water and soil, and can reportedly affect reproductive health in humans and animals. However, the effect of simazine on female germ cell development remains unclear. In the present study, we observed that simazine exposure decreased oocyte maturation competence and embryonic developmental capacity. Importantly, simazine exposure disrupted microtubule stability and actin polymerization, resulting in failure of spindle assembly and migration. In addition, simazine exposure impaired mitochondrial function and cytosolic Ca2+ homeostasis in both oocyte and 2-cell embryos, thus increasing the levels of reactive oxygen species (ROS). Moreover, simazine exposure induced DNA damage and early apoptosis during oocyte maturation. Collectively, our results demonstrate that simazine exposureinduced mitochondrial dysfunction and apoptosis are major causes of poor oocytes quality.