In Vitro Infectious Risk Assessment of Heliothis virescens ascovirus 3j (HvAV-3j) toward Non-target Vertebrate Cells
In Vitro Infectious Risk Assessment of Heliothis virescens ascovirus 3j (HvAV-3j) toward Non-target Vertebrate Cells
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烟夜蛾子囊病毒 3j (HvAV-3j) 对非靶脊椎动物细胞的体外感染风险评估
DOI：
10.1007/s12250-019-00113-4
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发表时间：
2019
影响因子：
5.5
通讯作者：
Guo-Hua Huang
中科院分区：
文献类型：
--
作者：
Huan Yu;Yi-Yi Ou-Yang;Ni Li;Madoka Nakai;Guo-Hua Huang
甜菜夜蛾（Spodoptera exigua）、甜菜夜蛾（S.斜纹夜蛾、棉铃虫和粘虫等的囊病毒适合于开发生物杀虫剂。本文研究了烟芽夜蛾3 j型病毒（HvAV-3 j）对昆虫和哺乳动物细胞的感染性。HvAV-3 j感染引起Sf 9、HzAM 1、SeFB和HaFB细胞的剧烈形态学变化，包括肿胀和脱离。值得注意的是，后一种现象在HvAV-3 j接种的哺乳动物细胞（HEK 293、7402、HePG 2、PK 15、ST和TM 3）中不发生。MTT法检测表明，HvAV-3 j对宿主昆虫细胞的生长有明显的抑制作用，但对哺乳动物细胞的生长无明显影响。此外，研究了病毒DNA复制、基因转录和蛋白质表达，结果一致表明HvAV-3 j病毒不能在非靶脊椎动物细胞中复制其基因组DNA、转录或表达其蛋白质。HvAV-3 j基因仅在四种昆虫细胞系中转录和表达。这些结果表明HvAV-3 j对S. frugiperda，S. exigua，小刺蛾H. armigera和H.但对人、猪和小鼠来源的细胞不敏感，表明囊病毒对非靶脊椎动物细胞是安全的。
As specific pathogens of noctuid pests, including Spodoptera exigua, S. litura, Helicoverpa armigera, and Mythimna separata, ascoviruses are suitable for the development of bioinsecticides. In this study, the infectivity of Heliothis virescens ascovirus 3j (HvAV-3j) on insect and mammalian cells was evaluated. HvAV-3j infection induced drastic morphological changes in Sf9, HzAM1, SeFB, and HaFB cells, including swelling and detachment. Notably, the latter phenomena did not occur in HvAV-3j-inoculated mammalian cells (HEK293, 7402, HePG2, PK15, ST, and TM3). MTT assays indicated that HvAV-3j inhibited the growth of host insect cells from the 6th hpi, but no effects were detected in the HvAV-3j-inoculated mammalian cells. Furthermore, viral DNA replication, gene transcription, and protein expression were investigated, and the results consistently suggested that HvAV-3j viruses were not able to replicate their genomic DNA, transcribe, or express their proteins in the non-target vertebrate cells. The HvAV-3j genes were only transcribed and expressed in the four insect cell lines. These results indicated that HvAV-3j was infectious to cells derived from S. frugiperda, S. exigua, H. armigera, and H. zea but not to cells derived from human, pig, and mouse, suggesting that ascoviruses are safe to non-target vertebrate cells.