Transcriptomic analysis of the orchestrated molecular mechanisms underlying fruiting body initiation in Chinese cordyceps.
Transcriptomic analysis of the orchestrated molecular mechanisms underlying fruiting body initiation in Chinese cordyceps.
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DOI：
10.1016/j.gene.2020.145061
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发表时间：
2020-08
期刊：
影响因子：
3.5
通讯作者：
Yanni Zhao;Jihong Zhang;Q. Meng;Huan Zhang;Guiling Zhou;Miaomiao Li;Pei-pei Wu;Ruihao Shu
中科院分区：
文献类型：
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作者：
Yanni Zhao;Jihong Zhang;Q. Meng;Huan Zhang;Guiling Zhou;Miaomiao Li;Pei-pei Wu;Ruihao Shu
冬虫夏草是冬虫夏草（Ophiocordyceps sinensis, sync . cordyceps sinensis）的子实体，是最有价值的中药真菌之一。o的转录组学分析。中华鲟揭示了其生命周期和生态重要性的几个方面。然而，子实体起始的分子机制尚不清楚。对子实体起始阶段菌丝、菌核和原基三个组织的发育转录组进行了分析。主成分分析表明，在三种组织中，基因表达模式存在差异。差异表达基因的功能分析表明，原基中DNA合成和细胞分裂活跃。此外，菌丝的功能是从环境中吸收某些物质，菌核是o的代谢中心。制成。参与丝裂原活化蛋白激酶（MAPK）信号通路的基因参与了子实体的起始。信息素受体基因（OSIN6252）和氨基酸感知基因（OSIN6398）两个环境感知基因在原基中高表达，提示它们在起始过程中起重要作用。这些结果提供了对不同基因的编排功能和基因谱的见解。在关键阶段的组织。这些发现将有助于揭示子实体起始的潜在机制，为进一步的人工栽培奠定基础。
Chinese cordyceps, the fruiting body of the Chinese caterpillar fungus (Ophiocordyceps sinensis, syn.Cordyceps sinensis), is among the most valuable traditional Chinese medicine fungi. Transcriptomic analysis ofO. sinensishas revealed several aspects of its life cycle and ecological importance. However, the molecular mechanisms involved in fruiting body initiation remain unclear. The developmental transcriptomes were analyzed from three tissues at the fruiting body initiation stage, namely, the mycelium, sclerotium and primordium. Principal component analysis showed that in the three tissues, the gene expression patterns differed from each other. The functional analysis of differentially expressed genes showed that DNA synthesis and cell division were active in the primordium. In addition, the function of the mycelium was to absorb certain substances from the environment and the sclerotium was the metabolism center ofO. sinensis. Genes participating in the mitogen-activated protein kinase (MAPK) signal pathway were involved in fruiting body initiation. Two environmental sensing genes, including a pheromone receptor gene (OSIN6252) and an amino acid sensing gene (OSIN6398), were highly expressed in the primordium, suggesting their important roles in initiation. These results provided insights into the orchestrated functions and gene profiles of differentO. sinensistissues at the key stage. These findings will aid in revealing the underlying mechanisms of fruiting body initiation, which will further benefit artificial cultivation.