An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
复制标题
DOI：
10.1038/s41467-019-11872-9
复制
发表时间：
2019-09-06
影响因子：
16.6
通讯作者：
Fu, Daolin
中科院分区：
文献类型：
--
作者：
Zhang, Chaozhong;Huang, Lin;Fu, Daolin
小麦条锈病是由小麦条锈菌（Pucciniastriiformis f.）小麦赤霉病菌（Pst）是小麦生产的全球性威胁。粗山羊草（Aegilops tauschii）是小麦的祖先之一，其4DS染色体上携带抗Pst的YrAS 2388位点。我们发现，YrAS 2388编码一个典型的核苷酸寡聚化结构域样受体（NLR）。抗Pst等位基因YrAS 2388 R具有重复的3'非翻译区，其特征在于核苷酸结合结构域中的选择性剪接。YrAS 2388 R等位基因的突变破坏了合成六倍体小麦对Pst的抗性; YrAS 2388 R转基因植株对普通小麦中的11个Pst小种和条锈菌的一个小种表现出抗性。大麦中的大麦斑点病菌（sp. hordei）。YrAS 2388 R等位基因仅存在于Ae. tauschii和Ae. tauschii衍生的合成小麦;它在100%（n = 461）测试的普通小麦品系中不存在。YrAS 2388 R的克隆将有助于小麦和其他小麦族物种的抗条锈病育种。
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production. Aegilops tauschii, one of the wheat progenitors, carries the YrAS2388 locus for resistance to Pst on chromosome 4DS. We reveal that YrAS2388 encodes a typical nucleotide oligomerization domain-like receptor ( NLR). The Pst-resistant allele YrAS2388R has duplicated 3' untranslated regions and is characterized by alternative splicing in the nucleotide-binding domain. Mutation of the YrAS2388R allele disrupts its resistance to Pst in synthetic hexaploid wheat; transgenic plants with YrAS2388R show resistance to eleven Pst races in common wheat and one race of P. striiformis f. sp. hordei in barley. The YrAS2388R allele occurs only in Ae. tauschii and the Ae. tauschii-derived synthetic wheat; it is absent in 100% (n = 461) of common wheat lines tested. The cloning of YrAS2388R will facilitate breeding for stripe rust resistance in wheat and other Triticeae species.