Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy
Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy
复制标题
用于酶前药癌症治疗的辣根过氧化物酶封装的壳聚糖纳米颗粒
DOI：
10.1007/s10529-014-1664-5
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发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Wang, Ping
中科院分区：
文献类型：
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作者：
Cao, Xiaodan;Chen, Chao;Wang, Ping
在各种基于酶的疗法中，酶前药物疗法(EPT)承诺将副作用降至最低，因为它在放置酶的地方激活无毒的前药物。这种方法的成功需要对结构变性和潜在的免疫原性都有很高的酶稳定性。这项工作检验了纳米粒子在EPT应用中用于酶保护的效率。具体地说，构建了辣根过氧化物酶(HRP)包裹的壳聚糖纳米粒(HRP-CSNP)，并检测了其稳定性。HRP-CSNP在37℃的变性剂尿素存在下保持了酶的活性，并提高了稳定性。这些纳米颗粒有效地结合在人乳腺癌细胞Bcap37的表面，当与前药吲哚-3-醋酸一起应用时，导致超过80%的细胞死亡。
Among various enzyme-based therapies, enzyme-prodrug therapy (EPT) promises minimized side effects in that it activates non-toxic prodrugs locally where the enzymes are placed. The success of such an approach requires high enzyme stability against both structural denaturation and potential immunogenicity. This work examines the efficiency of nanoparticles for enzyme protection in EPT applications. Specifically, horseradish peroxidase (HRP)-encapsulated chitosan nanoparticles (HRP-CSNP) were constructed and examined with respect to stability enhancement. HRP-CSNP retained enzyme activity and had improved stability at 37 °C in the presence of a denaturant, urea. The nanoparticles effectively bound to the surface of human breast cancer cell Bcap37 and led to over 80 % cell death when applied with a prodrug indole-3-acetic acid.